BMRB Entry 11597

Assembly:

Entities:

Entity 1, Monosiga brevicollis CRK/CRKL homolog (crka1) SH2 domain 104 residues - 11482.751 Da.

Residues -6 to 0 are cloning artifacts. GenBank entry KT795324 which corresponds to mRNA sequence of Monosiga brevicollis crka1 is not released yet. The nucleotide sequence of KT795324 is: TGCGCCTCGATCATTTTGAGCCCCAGGGCCTTTGTTGTGCATCATGGCGGAAGCCGCAGCACCCTGGTATCATGGGCCGC TGAGCCGCACCGATGCAGAAAACAGTCTCTTGCGTATGCCCGAAGGGACTTTCTTGGTCCGCGACAGCACCTCGTCCCCC GGAGATTATGTCCTCTCATGCAGCGAGAATGGCAAGGTGACGCACTACAAGCTCTCGGCCGAAGAGGGCAAGATTCGTAT CGATACACACCTCTTTGACAACCTGGATGCTGCCATCACCTTTTACATGGAGCACGAGTTGGAATACAGTTCGCTCAAAC AGCCCCTGCAACGGCAATCATCATGGCGCGATGACGAGCTTGTCGCCACGGTCATGTTCTCTGCAGAAGGGCTGTACAAG TTTGTCGGCCGCAATGAAAACGATTTACCCTTTGACAAGGGGGAGCTTCTCAATGTCTTGCAGCTAACACAGGACAATTG GTGGATGGCCCAGAGTCAGAAAACGCTCAAGATTGGCTACGTTCCCGTCAATTATGTCCGGGCGCTAGAGCAGCCAGCTG CCCCGCCACCGCGCCCTGCTCCACGTCCTGCCGAGGAGGAGTCTTCCAGGCTTGAGCGAACCGAATCCCGTCGTACGCCG GCCAGCCACCATCGACCCAAGGAACCCATGCCGGCAGGTGCCGTCATGACACGAGCTCCGGCTCGACATCACCAAGAGGC AAAGGCACCCCCTGCACGGCCTCAGCCGACTGGGCCTTCTGCCGCGGGTCCGATAGCCGCTGCTGCTCCGCCTCCACGTC CCAAAACATCAAAGCCCACGACCGACTTTAGTGCGCTCAAGGCTCAACTGCAGGAGCCAATTGTTTCCAATCCCACGCCC ACCTCGACGGCCCCAGAGCAGGCGGCTTTGCCCGCCGAAACCACGGCAGAAGTCTCAACACCAACGCAGCCGCTCGCATC TGCTCCGCCCACTTCTACGGCCTCGCATGAAGCGCCGGCAAGCTCTGCCGCACCCGCTACTCAGCACGGCGCAGGGTCAG AGGATCACGCTACCGCTCCCGACTCGGAGCCTCCCATCACTTTGGTCCGCGCCATCACCGCCATCATCCCCAACGCCTAT GACAATGATGCCTTGGTCTTGGAGGAAAATGATTTGGTTCACGTGCTGGATGGCGGCGAAGGCGCACTGGAGCGGGGCCG CGTTCTCAGAACTGGACAGATCGGCTTGTATCCCTTTGCAAAGACTGAGGATATTGAGAGCCACAACTACGATGCCGAAG TGAAGGCAGCCGTCGCTGACTACCTGAAGCGGTTTCCAGATGCATGCTAGCAAACCATAGGTGGCTATTGGCGACGGTCG TGAGATACGT And the translated protein sequence is: MAEAAAPWYHGPLSRTDAENSLLRMPEGTFLVRDSTSSPGDYVLSCSENGKVTHYKLSAEEGKIRIDTHLFDNLDAAITF YMEHELEYSSLKQPLQRQSSWRDDELVATVMFSAEGLYKFVGRNENDLPFDKGELLNVLQLTQDNWWMAQSQKTLKIGYV PVNYVRALEQPAAPPPRPAPRPAEEESSRLERTESRRTPASHHRPKEPMPAGAVMTRAPARHHQEAKAPPARPQPTGPSA AGPIAAAAPPPRPKTSKPTTDFSALKAQLQEPIVSNPTPTSTAPEQAALPAETTAEVSTPTQPLASAPPTSTASHEAPAS SAAPATQHGAGSEDHATAPDSEPPITLVRAITAIIPNAYDNDALVLEENDLVHVLDGGEGALERGRVLRTGQIGLYPFAK TEDIESHNYDAEVKAAVADYLKRFPDAC

Samples:

sample_1: crka1 SH2 domain, [U-13C; U-15N], 1.1 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; sodium azide 0.02%; DTT, [U-2H], 1 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

xwinnmr v3.6, Bruker Biospin - collection

TOPSPIN v2.1, Bruker Biospin - collection

NMRPipe v20110801, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.2.2_01, Johnson, One Moon Scientific - data analysis

Kujira v0.9843, Kobayashi, Iwahara, Koshiba, Tomizawa, Tochio, G ntert, Kigawa, Yokoyama - chemical shift assignment, data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

• Bruker Avance 600 MHz
• Bruker Avance 800 MHz