BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 1215

Title: Purification and characterization of a non-reconstitutable azurin, obtained by heterologous expression of the Pseudomonas aeruginosa azu gene in Escherichia coli

Deposition date: 1995-07-31 Original release date: 1995-07-31

Authors: van de Kamp, Mart; Hali, Frits; Rosato, Nicola; Finazzi Agro, Alessandro; Canters, Gerard

Citation: van de Kamp, Mart; Hali, Frits; Rosato, Nicola; Finazzi Agro, Alessandro; Canters, Gerard. "Purification and characterization of a non-reconstitutable azurin, obtained by heterologous expression of the Pseudomonas aeruginosa azu gene in Escherichia coli"  Biochim. Biophys. Acta 1019, 283-292 (1990).

Assembly members:
azurin, polymer, 128 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: not available

Entity Sequences (FASTA):
azurin: AECSVDIQGNDQMQFNTNAI TVDKSCKQFTVNLSHPGNLP KNVMGHNWVLSTAADMQGVV TDGMASGLDKDYLKPDDSRV IAHTKLIGSGEKDSVTFDVS KLKEGEQYMFFCTFPGHSAL MKGTLTLK

Data sets:
Data typeCount
1H chemical shifts15

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1azurin1

Entities:

Entity 1, azurin 128 residues - Formula weight is not available

1   ALAGLUCYSSERVALASPILEGLNGLYASN
2   ASPGLNMETGLNPHEASNTHRASNALAILE
3   THRVALASPLYSSERCYSLYSGLNPHETHR
4   VALASNLEUSERHISPROGLYASNLEUPRO
5   LYSASNVALMETGLYHISASNTRPVALLEU
6   SERTHRALAALAASPMETGLNGLYVALVAL
7   THRASPGLYMETALASERGLYLEUASPLYS
8   ASPTYRLEULYSPROASPASPSERARGVAL
9   ILEALAHISTHRLYSLEUILEGLYSERGLY
10   GLULYSASPSERVALTHRPHEASPVALSER
11   LYSLEULYSGLUGLYGLUGLNTYRMETPHE
12   PHECYSTHRPHEPROGLYHISSERALALEU
13   METLYSGLYTHRLEUTHRLEULYS

Samples:

sample_one:

sample_condition_set_one: pH: 10 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 1210 1211 1212 1213 1214 1216 1217 1218 1219 18254 4465
PDB
DBJ BAK87490 BAP24727 BAP53499 BAQ42761 BAR70381
EMBL CAA30279 CAW30062 CCQ86145 CDH73668 CDH79986
GB AAA25730 AAG08307 AAP03090 AAT49489 ABJ14307
PRF 671048A
REF NP_253609 WP_003095591 WP_003141697 WP_012077620 WP_031690945
SP B3EWN9 P00282