BMRB Entry 15046
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15046
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Title: 1H, 13C, and 15N resonance assignments of subunit F of the A1AO ATP synthase from Methanosarcina mazei Go1 PubMed: 19636817
Deposition date: 2006-11-21 Original release date: 2007-06-26
Authors: Gayen, Shovanlal; Vivekanandan, Subramanian; Biukovic, Goran; Gruber, Gerhard; Yoon, Ho Sup
Citation: Gayen, Shovanlal; Vivekanandan, Subramanian; Biukovic, Goran; Gruber, Gerhard; Yoon, Ho Sup. "1H, 13C, and 15N resonance assignments of subunit F of the A(1)A (O) ATP synthase from Methanosarcina mazei Go1" Biomol. NMR Assignments 1, 23-25 (2007).
Assembly members:
F_subunit_A_type_ATP_synthase, polymer, 110 residues, 11944.677 Da.
Natural source: Common Name: Methanosarcina mazei Go1 Taxonomy ID: 2209 Superkingdom: Archaea Kingdom: not available Genus/species: Methanosarcina mazei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
F_subunit_A_type_ATP_synthase: MKHHHHHHPMELAVIGKSEF
VTGFRLAGISKVYETPDIPA
TESAVRSVLEDKSVGILVMH
NDDIGNLPEVLRKNLNESVQ
PTVVALGGSGSGSTSLREKI
KQAVGVDLWK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 395 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 681 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit F | 1 |
Entities:
Entity 1, subunit F 110 residues - 11944.677 Da.
| 1 | MET | LYS | HIS | HIS | HIS | HIS | HIS | HIS | PRO | MET | |
| 2 | GLU | LEU | ALA | VAL | ILE | GLY | LYS | SER | GLU | PHE | |
| 3 | VAL | THR | GLY | PHE | ARG | LEU | ALA | GLY | ILE | SER | |
| 4 | LYS | VAL | TYR | GLU | THR | PRO | ASP | ILE | PRO | ALA | |
| 5 | THR | GLU | SER | ALA | VAL | ARG | SER | VAL | LEU | GLU | |
| 6 | ASP | LYS | SER | VAL | GLY | ILE | LEU | VAL | MET | HIS | |
| 7 | ASN | ASP | ASP | ILE | GLY | ASN | LEU | PRO | GLU | VAL | |
| 8 | LEU | ARG | LYS | ASN | LEU | ASN | GLU | SER | VAL | GLN | |
| 9 | PRO | THR | VAL | VAL | ALA | LEU | GLY | GLY | SER | GLY | |
| 10 | SER | GLY | SER | THR | SER | LEU | ARG | GLU | LYS | ILE | |
| 11 | LYS | GLN | ALA | VAL | GLY | VAL | ASP | LEU | TRP | LYS |
Samples:
sample_1: F subunit A type ATP synthase, [U-13C; U-15N], 0.1 – 0.5 mM
sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v1.3, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| GB | AAC06374 AAM30477 AGF96210 AKB39529 AKB60502 |
| REF | WP_011032731 WP_048048603 WP_054869506 |
| SP | Q60185 |
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