BMRB Entry 15055

Name: Structure for the N-terminus of chromosomal replication initiation protein dnaA from M. genitalium
Published: 2007-10-16

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PDB ID: 2jmp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15055
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structure for the N-terminus of chromosomal replication initiation protein dnaA from M. genitalium PubMed: 17680349

Deposition date: 2006-11-27 Original release date: 2007-10-16

Authors: Lowery, Thomas; Pelton, Jeffrey; Wemmer, David

Citation: Lowery, Thomas; Pelton, Jeffrey; Chandonia, John-Marc; Kim, Rosalind; Yokota, Hisao; Wemmer, David. "NMR Structure of the N-terminal domain of the replication initiator protein DnaA" J. Struct. Funct. Genomics 8, 11-17 (2007).

Assembly members:
N_dnaA, polymer, 100 residues, 11870.441 Da.

Natural source: Common Name: Mycoplasma genitalium Taxonomy ID: 2097 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycoplasma genitalium

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
N_dnaA: MEQFNAFKSLLKKHYEKTIG FHDKYIKDINRFVFKNNVLL ILLENEFARNSLNDNSEIIH LAESLYEGIKSVNFVNEQDF FFNLAKLEENSRDTLYQNSG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	403
15N chemical shifts	99
1H chemical shifts	602

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	N_dnaA	1

Samples:

sample_1: N_dnaA, [U-100% 15N], 0.75 ± 0.2 mM; sodium phosphate 50 ± 10 mM; sodium chloride 100 ± 10 mM; EDTA 2 ± 1 mM; sodium azide 0.05 ± 0.01 %

sample_2: N_dnaA, [U-98% 13C; U-98% 15N], 0.75 ± 0.2 mM; sodium phosphate 50 ± 10 mM; sodium chloride 100 ± 10 mM; EDTA 2 ± 1 mM; sodium azide 0.05 ± 0.01 %

sample_3: N_dnaA 0.75 ± 0.2 mM; sodium phosphate 50 ± 10 mM; sodium chloride 100 ± 10 mM; EDTA 2 ± 1 mM; sodium azide 0.05 ± 0.01 %

sample_4: N_dnaA, [U-98% 13C; U-98% 15N], 0.75 ± 0.2 mM; sodium phosphate 50 ± 10 mM; sodium chloride 100 ± 10 mM; EDTA 2 ± 1 mM; sodium azide 0.05 ± 0.01 %

sample_5: N_dnaA, [10% 13C], 0.75 ± 0.2 mM; sodium phosphate 50 ± 10 mM; sodium chloride 100 ± 10 mM; EDTA 2 ± 1 mM; sodium azide 0.05 ± 0.01 %

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3d HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1
4D HCCH NOESY	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
2D DQF-COSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1

1	MET	GLU	GLN	PHE	ASN	ALA	PHE	LYS	SER	LEU
2	LEU	LYS	LYS	HIS	TYR	GLU	LYS	THR	ILE	GLY
3	PHE	HIS	ASP	LYS	TYR	ILE	LYS	ASP	ILE	ASN
4	ARG	PHE	VAL	PHE	LYS	ASN	ASN	VAL	LEU	LEU
5	ILE	LEU	LEU	GLU	ASN	GLU	PHE	ALA	ARG	ASN
6	SER	LEU	ASN	ASP	ASN	SER	GLU	ILE	ILE	HIS
7	LEU	ALA	GLU	SER	LEU	TYR	GLU	GLY	ILE	LYS
8	SER	VAL	ASN	PHE	VAL	ASN	GLU	GLN	ASP	PHE
9	PHE	PHE	ASN	LEU	ALA	LYS	LEU	GLU	GLU	ASN
10	SER	ARG	ASP	THR	LEU	TYR	GLN	ASN	SER	GLY

PDB	2JMP
GB	AAC72490 ABY79668 AFQ03308 AFQ03792 AFQ04301
REF	WP_010869493 WP_014894707
SP	P35888

Biological Magnetic Resonance Data Bank

BMRB Entry 15055

Title: Structure for the N-terminus of chromosomal replication initiation protein dnaA from M. genitalium PubMed: 17680349

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLU	GLN	PHE	ASN	ALA	PHE	LYS	SER	LEU
2	LEU	LYS	LYS	HIS	TYR	GLU	LYS	THR	ILE	GLY
3	PHE	HIS	ASP	LYS	TYR	ILE	LYS	ASP	ILE	ASN
4	ARG	PHE	VAL	PHE	LYS	ASN	ASN	VAL	LEU	LEU
5	ILE	LEU	LEU	GLU	ASN	GLU	PHE	ALA	ARG	ASN
6	SER	LEU	ASN	ASP	ASN	SER	GLU	ILE	ILE	HIS
7	LEU	ALA	GLU	SER	LEU	TYR	GLU	GLY	ILE	LYS
8	SER	VAL	ASN	PHE	VAL	ASN	GLU	GLN	ASP	PHE
9	PHE	PHE	ASN	LEU	ALA	LYS	LEU	GLU	GLU	ASN
10	SER	ARG	ASP	THR	LEU	TYR	GLN	ASN	SER	GLY

1	MET	GLU	GLN	PHE	ASN	ALA	PHE	LYS	SER	LEU
2	LEU	LYS	LYS	HIS	TYR	GLU	LYS	THR	ILE	GLY
3	PHE	HIS	ASP	LYS	TYR	ILE	LYS	ASP	ILE	ASN
4	ARG	PHE	VAL	PHE	LYS	ASN	ASN	VAL	LEU	LEU
5	ILE	LEU	LEU	GLU	ASN	GLU	PHE	ALA	ARG	ASN
6	SER	LEU	ASN	ASP	ASN	SER	GLU	ILE	ILE	HIS
7	LEU	ALA	GLU	SER	LEU	TYR	GLU	GLY	ILE	LYS
8	SER	VAL	ASN	PHE	VAL	ASN	GLU	GLN	ASP	PHE
9	PHE	PHE	ASN	LEU	ALA	LYS	LEU	GLU	GLU	ASN
10	SER	ARG	ASP	THR	LEU	TYR	GLN	ASN	SER	GLY

1	MET	GLU	GLN	PHE	ASN	ALA	PHE	LYS	SER	LEU
2	LEU	LYS	LYS	HIS	TYR	GLU	LYS	THR	ILE	GLY
3	PHE	HIS	ASP	LYS	TYR	ILE	LYS	ASP	ILE	ASN
4	ARG	PHE	VAL	PHE	LYS	ASN	ASN	VAL	LEU	LEU
5	ILE	LEU	LEU	GLU	ASN	GLU	PHE	ALA	ARG	ASN
6	SER	LEU	ASN	ASP	ASN	SER	GLU	ILE	ILE	HIS
7	LEU	ALA	GLU	SER	LEU	TYR	GLU	GLY	ILE	LYS
8	SER	VAL	ASN	PHE	VAL	ASN	GLU	GLN	ASP	PHE
9	PHE	PHE	ASN	LEU	ALA	LYS	LEU	GLU	GLU	ASN
10	SER	ARG	ASP	THR	LEU	TYR	GLN	ASN	SER	GLY