BMRB Entry 15095

Name: Solution Structure of a Dodecapeptide from Alpha-Synuclein Bound with Synphilin-1
Published: 2009-09-29

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PDB ID: 2jn5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15095
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution Structure of a Dodecapeptide from Alpha-Synuclein Bound with Synphilin-1 PubMed: 19762560

Deposition date: 2007-01-04 Original release date: 2009-09-29

Authors: Zhou, Chen-Jie; Hu, Hong-Yu; Lin, Dong-Hai

Citation: Xie, Yuan-Yuan; Zhou, Chen-Jie; Zhou, Zi-Ren; Hong, Jing; Che, Mei-Xia; Fu, Qing-Shan; Song, Ai-Xin; Lin, Dong-Hai; Hu, Hong-Yu. "Interaction with synphilin-1 promotes inclusion formation of alpha-synuclein: mechanistic insights and pathological implication." FASEB J. 24, 196-205 (2010).

Assembly members:
Alpha-Syn12, polymer, 12 residues, 1357.708 Da.
Synphilin, non-polymer, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
Alpha-Syn12: MDVFMKGLSKAK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Alpha-Syn12	1
2	Synphilin-1	2

Entities:

Entity 1, Alpha-Syn12 12 residues - 1357.708 Da.

1

MET

ASP

VAL

PHE

MET

LYS

GLY

LEU

SER

LYS

2

ALA

LYS

Entity 2, Synphilin-1 - . - Formula weight is not available

1

Synphilin

Samples:

sample_1: entity 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; Synphilin-1 0.1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - chemical shift assignment, peak picking

ARIA v2.0, Linge, O, . - data analysis, refinement, structure solution

NMR spectrometers:

Varian INOVA 600 MHz