BMRB Entry 15123
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15123
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Title: SeV Ntail(443-501) PubMed: 17459940
Deposition date: 2007-02-01 Original release date: 2007-05-14
Authors: Houben, Klaartje; Marion, Dominique; Tarbouriech, Nicolas; Ruigrok, Rob; Blanchard, Laurence
Citation: Houben, Klaartje; Marion, Dominique; Tarbouriech, Nicolas; Ruigrok, Rob; Blanchard, Laurence. "Interaction of the C-terminal domains of sendai virus N and P proteins: comparison of polymerase-nucleocapsid interactions within the paramyxovirus family" J. Virol. 81, 6807-6816 (2007).
Assembly members:
SeV_nucleoprotein_Ntail, polymer, 59 residues, Formula weight is not available
Natural source: Common Name: Respirovirus Taxonomy ID: 186938 Superkingdom: Viruses Kingdom: not available Genus/species: Respirovirus not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SeV_nucleoprotein_Ntail: GESGERWARQVSGGHFVTLH
GAERLEEETNDEDVSDIERR
IAMRLAERRQEDSATHGDE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 162 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 357 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ntail | 1 |
Entities:
Entity 1, Ntail 59 residues - Formula weight is not available
| 1 | GLY | GLU | SER | GLY | GLU | ARG | TRP | ALA | ARG | GLN | ||||
| 2 | VAL | SER | GLY | GLY | HIS | PHE | VAL | THR | LEU | HIS | ||||
| 3 | GLY | ALA | GLU | ARG | LEU | GLU | GLU | GLU | THR | ASN | ||||
| 4 | ASP | GLU | ASP | VAL | SER | ASP | ILE | GLU | ARG | ARG | ||||
| 5 | ILE | ALA | MET | ARG | LEU | ALA | GLU | ARG | ARG | GLN | ||||
| 6 | GLU | ASP | SER | ALA | THR | HIS | GLY | ASP | GLU |
Samples:
13C_15N: SeV nucleoprotein Ntail, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 500 mM; potassium phosphate 50 mM
sample_condition_1: ionic strength: 0.5 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | 13C_15N | isotropic | sample_condition_1 |
| 3D CBCA(CO)NH | 13C_15N | isotropic | sample_condition_1 |
| 3D HCCH-TOCSY | 13C_15N | isotropic | sample_condition_1 |
| 3D C(CO)NH | 13C_15N | isotropic | sample_condition_1 |
| 3D HNCACB | 13C_15N | isotropic | sample_condition_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian Avance 600 MHz
- Varian Avance 800 MHz
Related Database Links:
| DBJ | BAD74219 BAD74225 BAF73479 BAF73485 BAH59027 |
| EMBL | CAA24945 CAA35092 CAA35715 |
| GB | AAB06278 AAB06284 AAB06290 |
| SP | P04857 P04858 Q07097 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts