BMRB Entry 15204
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15204
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Title: Backbone assignment of human IgG1 CH3 domain PubMed: 19636836
Deposition date: 2007-04-05 Original release date: 2007-08-23
Authors: Liu, Dingjiang; Cocco, Melanie; Remmele, Richard; Brems, David
Citation: Liu, Dingjiang; Cocco, Melanie; Matsumura, Masazumi; Ren, Da; Becker, Bridget; Remmele, Richard; Brems, David. "Assignment of 1H, 13C and 15N resonances of the reduced human IgG1 C(H)3 domain" Biomol. NMR Assignments 1, 93-94 (2007).
Assembly members:
human_IgG1_CH3_domain, polymer, 107 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
human_IgG1_CH3_domain: GQPREPQVYTLPPSRDELTK
NQVSLTCLVKGFYPSDIAVE
WESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQG
NVFSCSVMHEALHNHYTQKS
LSLSPGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 394 |
| 15N chemical shifts | 110 |
| 1H chemical shifts | 610 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CH3 dimer chain 1 | 1 |
| 2 | CH3 dimer chain 2 | 1 |
Entities:
Entity 1, CH3 dimer chain 1 107 residues - Formula weight is not available
| 1 | GLY | GLN | PRO | ARG | GLU | PRO | GLN | VAL | TYR | THR | ||||
| 2 | LEU | PRO | PRO | SER | ARG | ASP | GLU | LEU | THR | LYS | ||||
| 3 | ASN | GLN | VAL | SER | LEU | THR | CYS | LEU | VAL | LYS | ||||
| 4 | GLY | PHE | TYR | PRO | SER | ASP | ILE | ALA | VAL | GLU | ||||
| 5 | TRP | GLU | SER | ASN | GLY | GLN | PRO | GLU | ASN | ASN | ||||
| 6 | TYR | LYS | THR | THR | PRO | PRO | VAL | LEU | ASP | SER | ||||
| 7 | ASP | GLY | SER | PHE | PHE | LEU | TYR | SER | LYS | LEU | ||||
| 8 | THR | VAL | ASP | LYS | SER | ARG | TRP | GLN | GLN | GLY | ||||
| 9 | ASN | VAL | PHE | SER | CYS | SER | VAL | MET | HIS | GLU | ||||
| 10 | ALA | LEU | HIS | ASN | HIS | TYR | THR | GLN | LYS | SER | ||||
| 11 | LEU | SER | LEU | SER | PRO | GLY | LYS |
Samples:
sample_1: human IgG1 CH3 domain, [U-100% 13C; U-100% 15N], 0.5 mM; sodium acetate 10 mM
sample_conditions_1: ionic strength: 0.02 M; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAA43606 CAM91874 |
| GB | AAD38158 AAF42993 AAG00448 ABD72617 ABQ01159 |
| PIR | A36040 S69339 S69340 |
| PRF | 1605217A |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts