BMRB Entry 15208

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PDB ID: 2jox
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15208
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed Beta-Sheet and Zinc Binuclear Cluster PubMed: 17610897

Deposition date: 2007-04-07 Original release date: 2008-05-06

Authors: Lee, Brian; Buck-Koehntop, Bethany; Martinez-Yamout, Maria; Gottesfeld, Joel; Dyson, H. Jane; Wright, Peter

Citation: Lee, Brian; Buck-Koehntop, Bethany; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed Beta-Sheet and Zinc Binuclear Cluster" J. Mol. Biol. 371, 1274-1289 (2007).

Assembly members:
Churchill, polymer, 106 residues, 12166.857 Da.
ZN, non-polymer, 65.409 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Churchill: CGDCVEKEYPNRGNTCLENG SFLLNFTGCAVCSKRDFMLI TNKSLKEEDGEEIVTYDHLC KNCHHVIARHEYTFSIMDEF QEYTMLCLLCGKAEDTISIL PDDPRQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	454
15N chemical shifts	113
1H chemical shifts	734

Additional metadata:

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Entity Assembly ID	Entity Name	Entity ID
1	protein	1
2	zinc ion, 1	2
3	zinc ion, 2	2
4	zinc ion, 3	2

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D (HCA)CO(CA)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D CBCGCD	sample_1	isotropic	sample_conditions_1
3D CBCGCE	sample_1	isotropic	sample_conditions_1
3D HNHB	sample_1	isotropic	sample_conditions_1
3D HACAHB-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

PDB	2JOX
DBJ	BAG51943 BAG59416 BAH12268
EMBL	CAH92555
GB	AAH20550 AAI68770 AIC61091 EAW80884 EAW80885
REF	NP_001100211 NP_001126496 NP_001189488 NP_001190992 NP_660148
SP	Q5R6Q5 Q8WUH1

Biological Magnetic Resonance Data Bank

BMRB Entry 15208

Title: Embryonic Neural Inducing Factor Churchill is not a DNA-Binding Zinc Finger Protein: Solution Structure Reveals a Solvent-Exposed Beta-Sheet and Zinc Binuclear Cluster PubMed: 17610897

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