BMRB Entry 15363
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15363
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Title: A D-amino acid containing conopeptide, marmophine, from Conus marmoreus PubMed: 18355315
Deposition date: 2007-07-04 Original release date: 2008-06-25
Authors: Huang, F.; Du, W.; Han, Y.; Wang, C.
Citation: Han, Y.; Huang, F.; Jiang, H.; Liu, L.; Wang, Q.; Wang, Y.; Shao, X.; Chi, C.; Du, W.; Wang, C.. "Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus" FEBS. J. 275, 1976-1987 (2008).
Assembly members:
M-conotoxin_mr12, polymer, 15 residues, 1935.080 Da.
Natural source: Common Name: Conus marmoreus Taxonomy ID: 42752 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus marmoreus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
M-conotoxin_mr12: DWEYHAHPKXNSXWT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 82 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chain A | 1 |
Entities:
Entity 1, chain A 15 residues - 1935.080 Da.
| 1 | ASP | TRP | GLU | TYR | HIS | ALA | HIS | PRO | LYS | HYP | ||||
| 2 | ASN | SER | DPN | TRP | THR |
Samples:
sample_1: M-conotoxin mr12 2.0 mM; TFA 0.01%; H2O 90%; D2O 10%
sample_2: M-conotoxin mr12 2.0 mM; TFA 0.01%; D2O 99.99%
sample_conditions_1: pH: 3; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D TOCSY | sample_1 | isotropic | sample_conditions_1 |
| DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D TOCSY | sample_2 | isotropic | sample_conditions_1 |
| DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker Inc. - collection
TOPSPIN v1.3B, Bruker Inc. - processing
SPARKY v3.110, T. D. Goddard and D. G. Kneller - structure solution
CYANA v2.1, L. A. Systems, Inc. - structure solution
AMBER v9.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Koll - refinement
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB |