BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15390

Title: Influenza Hemagglutinin Fusion Domain Mutant F9A   PubMed: 17567572

Deposition date: 2007-07-18 Original release date: 2008-06-18

Authors: Lai, Alex; Tamm, Lukas

Citation: Lai, Alex; Tamm, Lukas. "Locking the kink in the influenza hemagglutinin fusion domain structure."  J. Biol. Chem. 282, 23946-23956 (2007).

Assembly members:
HA_FP_F9A, polymer, 27 residues, 1978.201 Da.

Natural source:   Common Name: Influenza virus   Taxonomy ID: 11309   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available Influenza Virus A

Experimental source:   Production method: chemical synthesis   Host organism: N.A.

Entity Sequences (FASTA):
HA_FP_F9A: GLFGAIAGAIENGWEGMIDG GCGKKKK

Data sets:
Data typeCount
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HA FP F9A1

Entities:

Entity 1, HA FP F9A 27 residues - 1978.201 Da.

1   GLYLEUPHEGLYALAILEALAGLYALAILE
2   GLUASNGLYTRPGLUGLYMETILEASPGLY
3   GLYCYSGLYLYSLYSLYSLYS

Samples:

sample_1: HA_FP_F9A peptide 2 mM; d38-DPC, [U-2H], 200 mM; MES 5 mM; HEPES 10 mM; DTT 5 mM; NaN3 0.05 % (w:v)

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

DYANA, Guntert, Braun and Wuthrich - structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

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