BMRB Entry 15419
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15419
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Title: Solution Structure of protein RPA3401, Northeast Structural Genomics Consortium Target RpT7, Ontario Center for Structural Proteomics Target RP3384
Deposition date: 2007-08-07 Original release date: 2007-08-13
Authors: Ignatchenko, Alexandr; Gutmanas, Aleksandras; Lemak, Alexander; Yee, Adelinda; Karra, Murthy; Srisailam, Sampath; Arrowsmith, Cheryl
Citation: Ignatchenko, Alexandr; Gutmanas, Aleksandras; Lemak, Alexander; Yee, Adelinda; Karra, Murthy; Srisailam, Sampath; Arrowsmith, Cheryl. "Solution Structure of protein RPA3401, Northeast Structural Genomics Consortium Target RpT7, Ontario Center for Structural Proteomics Target RP3384" The BMRB entry is the only known published source for the data..
Assembly members:
RPA3401, polymer, 65 residues, 7380.308 Da.
Natural source: Common Name: Rhodopseudomonas palustris Taxonomy ID: 1076 Superkingdom: Bacteria Kingdom: not available Genus/species: Rhodopseudomonas palustris
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RPA3401: MATADDFKLIRDIHSTGGRR
QVFGSREQKPFEDLVDLGWL
KRSSVDSRATHYQITERGTS
AALRS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 249 |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 400 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RPA3401 | 1 |
Entities:
Entity 1, RPA3401 65 residues - 7380.308 Da.
| 1 | MET | ALA | THR | ALA | ASP | ASP | PHE | LYS | LEU | ILE | ||||
| 2 | ARG | ASP | ILE | HIS | SER | THR | GLY | GLY | ARG | ARG | ||||
| 3 | GLN | VAL | PHE | GLY | SER | ARG | GLU | GLN | LYS | PRO | ||||
| 4 | PHE | GLU | ASP | LEU | VAL | ASP | LEU | GLY | TRP | LEU | ||||
| 5 | LYS | ARG | SER | SER | VAL | ASP | SER | ARG | ALA | THR | ||||
| 6 | HIS | TYR | GLN | ILE | THR | GLU | ARG | GLY | THR | SER | ||||
| 7 | ALA | ALA | LEU | ARG | SER |
Samples:
sample_1: RHA3401, [U-13C; U-15N], 0.6 mM; NaCl 300 mM; TRIS, [U-99% 2H], 10 mM; Benzamidine 1 mM; NaN3 0.01%
sample_2: RHA3401, [U-13C; U-15N], 0.6 mM; NaCl 300 mM; TRIS, [U-99% 2H], 10 mM; Benzamidine 1 mM; NaN3 0.01%
sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D H(C)CH TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 4D(PR) HBHACBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 4D(PR) HCC(CO)NH TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C aromatic NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TALOS, Cornilescu, Delaglio and Bax - data analysis
SPARKY, Goddard - peak picking
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts