BMRB Entry 15435
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15435
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Title: alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR PubMed: 18295795
Deposition date: 2007-08-15 Original release date: 2008-03-13
Authors: Feng, Zhi-Ping
Citation: Ellison, Michael; Feng, Zhi-Ping; Park, Anthony; Zhang, Xuecheng; Olivera, Baldomero; McIntosh, J. Michael; Norton, Raymond. "alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR: Structure and Identification of Key Receptor-Binding Residues." J. Mol. Biol. 377, 1216-1227 (2008).
Assembly members:
RgIA, polymer, 13 residues, 1579.867 Da.
Natural source: Common Name: Conus regius Taxonomy ID: 101314 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus regius
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
RgIA: GCCSDPRCRYRCR
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 33 |
15N chemical shifts | 11 |
1H chemical shifts | 78 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RgIA | 1 |
Entities:
Entity 1, RgIA 13 residues - 1579.867 Da.
1 | GLY | CYS | CYS | SER | ASP | PRO | ARG | CYS | ARG | TYR | ||||
2 | ARG | CYS | ARG |
Samples:
sample_1: toxin 1.0 ± 0.2 mM; H2O 95%; D2O 5%
sample_conditions_2: pH: 5.0; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_2 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_2 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_2 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_2 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts