BMRB Entry 15447
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15447
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Title: 8F19F1 module pair from Fibronectin PubMed: 17921136
Deposition date: 2007-08-29 Original release date: 2007-10-08
Authors: Millard, Chris; Campbell, Iain; Ellis, Ian; Pickford, Andrew; Schor, Anna
Citation: Millard, Chris; Ellis, Ian; Pickford, Andrew; Schor, Ana; Schor, Seth; Campbell, Iain. "The role of the fibronectin IGD motif in stimulating fibroblast migration" J. Biol. Chem. 282, 35530-35535 (2007).
Assembly members:
8F19F1, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
8F19F1: DQCIVDDITYNVNDTFHKKH
EEGHMLNCTCFGQGRGRWKC
DPVDQCQDSETGTFYQIGDS
WEKYVHGVRYQCYCYGRGIG
EWHCQPLQ
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 83 |
1H chemical shifts | 90 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 8F19F1 | 1 |
Entities:
Entity 1, 8F19F1 88 residues - Formula weight is not available
1 | ASP | GLN | CYS | ILE | VAL | ASP | ASP | ILE | THR | TYR | ||||
2 | ASN | VAL | ASN | ASP | THR | PHE | HIS | LYS | LYS | HIS | ||||
3 | GLU | GLU | GLY | HIS | MET | LEU | ASN | CYS | THR | CYS | ||||
4 | PHE | GLY | GLN | GLY | ARG | GLY | ARG | TRP | LYS | CYS | ||||
5 | ASP | PRO | VAL | ASP | GLN | CYS | GLN | ASP | SER | GLU | ||||
6 | THR | GLY | THR | PHE | TYR | GLN | ILE | GLY | ASP | SER | ||||
7 | TRP | GLU | LYS | TYR | VAL | HIS | GLY | VAL | ARG | TYR | ||||
8 | GLN | CYS | TYR | CYS | TYR | GLY | ARG | GLY | ILE | GLY | ||||
9 | GLU | TRP | HIS | CYS | GLN | PRO | LEU | GLN |
Samples:
sample_1: 8F19F1, [U-15N], 1 mM; D2O, [U-2H], 5%; sodium azide 0.02%; potassium phosphate 20 mM; sodium chloride 150 mM; dioxane 1 mM
sample_2: 8F19F1 1 mM; sodium azide 0.02%; potassium phosphate 20 mM; sodium chloride 150 mM; dioxane 1 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.6; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian OMEGA 600 MHz
- Varian OMEGA 500 MHz
- Varian OMEGA 750 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts