BMRB Entry 15448
Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR15448
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for ClpS from Xanthomonas axonopodis pv citri
Deposition date: 2007-08-29 Original release date: 2007-10-01
Authors: Galvao-Botton, Leonor; Farah, Chuck; Spisni, Alberto; Pertinhez, Thelma
Citation: Galvao-Botton, Leonor; Spisni, Alberto; Salinas, Roberto; Farah, Chuck; Pertinhez, Thelma. "NMR structural investigations of the ClpS protein from Xanthomonas axonopodis pv citri" Not known ., .-..
Assembly members:
ClpS, polymer, 106 residues, 12120.8 Da.
Natural source: Common Name: Xanthomonas axonopodis pv citri Taxonomy ID: 53413 Superkingdom: Bacteria Kingdom: not available Genus/species: Xanthomonas axonopodis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ClpS: MPRNTSHEHDHGLMVEASKP
EVAPPPRYQVLLLNDDYTPM
DFVVTVLEQFFNLNLEQATQ
IMLHVHTRGRGVCGVYSREV
AESKVAQVNEFSRMNQHPLL
CTMEQA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 368 |
15N chemical shifts | 95 |
1H chemical shifts | 537 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ClpS | 1 |
Entities:
Entity 1, ClpS 106 residues - 12120.8 Da.
1 | MET | PRO | ARG | ASN | THR | SER | HIS | GLU | HIS | ASP | ||||
2 | HIS | GLY | LEU | MET | VAL | GLU | ALA | SER | LYS | PRO | ||||
3 | GLU | VAL | ALA | PRO | PRO | PRO | ARG | TYR | GLN | VAL | ||||
4 | LEU | LEU | LEU | ASN | ASP | ASP | TYR | THR | PRO | MET | ||||
5 | ASP | PHE | VAL | VAL | THR | VAL | LEU | GLU | GLN | PHE | ||||
6 | PHE | ASN | LEU | ASN | LEU | GLU | GLN | ALA | THR | GLN | ||||
7 | ILE | MET | LEU | HIS | VAL | HIS | THR | ARG | GLY | ARG | ||||
8 | GLY | VAL | CYS | GLY | VAL | TYR | SER | ARG | GLU | VAL | ||||
9 | ALA | GLU | SER | LYS | VAL | ALA | GLN | VAL | ASN | GLU | ||||
10 | PHE | SER | ARG | MET | ASN | GLN | HIS | PRO | LEU | LEU | ||||
11 | CYS | THR | MET | GLU | GLN | ALA |
Samples:
sample_1: ClpS, [U-99% 13C; U-99% 15N], 0.5 mM; D2O 5%; sodium phosphate 40 mM; sodium azide 0.025%
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView v5.0, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 500 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts