BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15485

Title: Segmental isotope labeling of Npl3   PubMed: 17936301

Deposition date: 2007-09-24 Original release date: 2008-06-26

Authors: Skrisovska, Lenka; Allain, Frederic

Citation: Skrisovska, Lenka; Allain, Frederic. "Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L."  J. Mol. Biol. 375, 151-164 (2008).

Assembly members:
Npl3_RRM1, polymer, 108 residues, 12239 Da.

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Npl3_RRM1: MGSSHHHHHHSSGLVPRGSH MHHRQEGELSNTRLFVRPFP LDVQESELNEIFGPFGPMKE VKILNGFAFVEFEEAESAAK AIEEVHGKSFANQPLEVVYS KLPAKRYR

Data sets:
Data typeCount
13C chemical shifts237
15N chemical shifts73
1H chemical shifts513

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Related Database Links:

PDB 2JVO 2OSQ
EMBL CAA46817 CAA50291 CAY78932
GB AAA34818 AAB64865 AHY75384 AJP38110 AJU58232
REF NP_010720
SP Q01560
TPG DAA12270

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts