BMRB Entry 15529
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15529
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Title: Back bone chemical shifts of B. subtillis protein YkuJ, Northeast Structural Genomics Consortium Target SR360. PubMed: 18436958
Deposition date: 2007-10-17 Original release date: 2007-10-31
Authors: Wang, Xu; Ho, Chi-Kent; Janjua, Haleema; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Bansal, Sonal; Prestegard, James
Citation: Wang, Xu; Bansal, Sonal; Jiang, Mei; Prestegard, James. "RDC-assisted modeling of symmetric protein homo-oligomers" Protein. Sci. 17, 899-907 (2008).
Assembly members:
SR360, polymer, 87 residues, 10320.8 Da.
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SR360: MSQLMGIITRLQSLQETAEA
ANEPMQRYFEVNGEKICSVK
YFEKNQTFELTVFQKGEKPN
TYPFDNIDMVSIEIFELLQL
EHHHHHH
- assigned_chemical_shifts
- RDCs
Data type | Count |
13C chemical shifts | 137 |
15N chemical shifts | 70 |
1H chemical shifts | 70 |
residual dipolar couplings | 111 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | SR360 | 1 |
Entities:
Entity 1, SR360 87 residues - 10320.8 Da.
Last 8 residues are part of the His tag
1 | MET | SER | GLN | LEU | MET | GLY | ILE | ILE | THR | ARG | ||||
2 | LEU | GLN | SER | LEU | GLN | GLU | THR | ALA | GLU | ALA | ||||
3 | ALA | ASN | GLU | PRO | MET | GLN | ARG | TYR | PHE | GLU | ||||
4 | VAL | ASN | GLY | GLU | LYS | ILE | CYS | SER | VAL | LYS | ||||
5 | TYR | PHE | GLU | LYS | ASN | GLN | THR | PHE | GLU | LEU | ||||
6 | THR | VAL | PHE | GLN | LYS | GLY | GLU | LYS | PRO | ASN | ||||
7 | THR | TYR | PRO | PHE | ASP | ASN | ILE | ASP | MET | VAL | ||||
8 | SER | ILE | GLU | ILE | PHE | GLU | LEU | LEU | GLN | LEU | ||||
9 | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: SR360, [U-100% 13C; U-100% 15N], 1.2 ± 0.1 mM; D2O 5%; DTT 100 mM; MES 20 mM; sodium chloride 100 mM; Calcium Chloride 5 mM
sample_2: SR360, [5% 13C; U-100% 15N], 1.2 ± 0.1 mM; D2O 5%; DTT 100 mM; MES 20 mM; sodium chloride 100 mM; Calcium Chloride 5 mM; pentaethylene glycol monododecyl ether 0.04 v/v; Hexanol 0.006 v/v
sample_3: SR360, [5% 13C; U-100% 15N], 1.2 ± 0.1 mM; D2O 5%; DTT 100 mM; MES 20 mM; sodium chloride 100 mM; Calcium Chloride 5 mM; Pf1 phage 0.01 w/v
sample_conditions_1: ionic strength: 0.115 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
2D IPAP 1H,15N-HSQC | sample_2 | anisotropic | sample_conditions_1 |
2D IPAP 1H-15N HSQC | sample_3 | anisotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
REDCAT, Valafar,Losonszi,Prestegard - data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
DBJ | BAI85020 BAM52055 BAM57632 GAK78526 |
EMBL | CAA10873 CAB13283 CBI42612 CCF04895 CCG49344 |
GB | ABS73750 ADM37497 ADV96428 AEB24186 AEB63055 |
REF | NP_389293 WP_003154613 WP_003218671 WP_010334055 WP_013352074 |
SP | O34588 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts