BMRB Entry 15626
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15626
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Title: backbone assignment of yeast (Saccharomyces cerevisiae) ARF1 ,non-myristoylated, GDP-bound PubMed: 18406649
Deposition date: 2008-01-15 Original release date: 2008-06-30
Authors: Liu, Yizhou
Citation: Liu, Yizhou; Prestegard, James. "Direct measurement of dipole-dipole/CSA cross-correlated relaxation by a constant-time experiment" J. Magn. Reson. 193, 23-31 (2008).
Assembly members:
yeast_ARF1, polymer, 188 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
yeast_ARF1: GLFASKLFSNLFGNKEMRIL
MVGLDGAGKTTVLYKLKLGE
VITTIPTIGFNVETVQYKNI
SFTVWDVGGQDRIRSLWRHY
YRNTEGVIFVVDSNDRSRIG
EAREVMQRMLNEDELRNAAW
LVFANKQDLPEAMSAAEITE
KLGLHSIRNRPWFIQATCAT
SGEGLYEGLEWLSNSLKNST
LEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 299 |
| 15N chemical shifts | 155 |
| 1H chemical shifts | 155 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | yeast ARF1 | 1 |
Entities:
Entity 1, yeast ARF1 188 residues - Formula weight is not available
| 1 | GLY | LEU | PHE | ALA | SER | LYS | LEU | PHE | SER | ASN | ||||
| 2 | LEU | PHE | GLY | ASN | LYS | GLU | MET | ARG | ILE | LEU | ||||
| 3 | MET | VAL | GLY | LEU | ASP | GLY | ALA | GLY | LYS | THR | ||||
| 4 | THR | VAL | LEU | TYR | LYS | LEU | LYS | LEU | GLY | GLU | ||||
| 5 | VAL | ILE | THR | THR | ILE | PRO | THR | ILE | GLY | PHE | ||||
| 6 | ASN | VAL | GLU | THR | VAL | GLN | TYR | LYS | ASN | ILE | ||||
| 7 | SER | PHE | THR | VAL | TRP | ASP | VAL | GLY | GLY | GLN | ||||
| 8 | ASP | ARG | ILE | ARG | SER | LEU | TRP | ARG | HIS | TYR | ||||
| 9 | TYR | ARG | ASN | THR | GLU | GLY | VAL | ILE | PHE | VAL | ||||
| 10 | VAL | ASP | SER | ASN | ASP | ARG | SER | ARG | ILE | GLY | ||||
| 11 | GLU | ALA | ARG | GLU | VAL | MET | GLN | ARG | MET | LEU | ||||
| 12 | ASN | GLU | ASP | GLU | LEU | ARG | ASN | ALA | ALA | TRP | ||||
| 13 | LEU | VAL | PHE | ALA | ASN | LYS | GLN | ASP | LEU | PRO | ||||
| 14 | GLU | ALA | MET | SER | ALA | ALA | GLU | ILE | THR | GLU | ||||
| 15 | LYS | LEU | GLY | LEU | HIS | SER | ILE | ARG | ASN | ARG | ||||
| 16 | PRO | TRP | PHE | ILE | GLN | ALA | THR | CYS | ALA | THR | ||||
| 17 | SER | GLY | GLU | GLY | LEU | TYR | GLU | GLY | LEU | GLU | ||||
| 18 | TRP | LEU | SER | ASN | SER | LEU | LYS | ASN | SER | THR | ||||
| 19 | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: yeast ARF1, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
VNMRJ, Varian - collection
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 15809 |
| PDB | |
| DBJ | GAA22060 |
| EMBL | CAA58255 CAA98769 CAG60356 CAY78316 CCC67945 |
| GB | AAA34431 AHY74824 AJP37565 AJU57677 AJU58380 |
| REF | NP_010089 XP_001646910 XP_003667691 XP_003674325 XP_003676799 |
| SP | P11076 |
| TPG | DAA11671 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts