BMRB Entry 15646
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15646
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR analysis of the closed conformation of syntaxin-1 PubMed: 18458823
Deposition date: 2008-02-02 Original release date: 2008-02-05
Authors: Chen, Xiaocheng; Lu, Jun; Dulubova, Irina
Citation: Chen, Xiaocheng; Lu, Jun; Dulubova, Irina; Rizo, Josep. "NMR analysis of the closed conformation of syntaxin-1" J. Biomol. NMR 41, 43-54 (2008).
Assembly members:
sx23, polymer, 207 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
sx23: GSRDRFMDEFFEQVEEIRGF
IDKIAENVEEVKRKHSAILA
SPNPDEKTKEELEELMSDIK
KTANKVRSKLKSIEQSIEQE
EGLNRSSADLRIRKTQHSTL
SRKFVEVMSEYNATQSDYRE
RCKGRIQRQLEITGRTTTSE
ELEDMLESGNPAIFASGIIM
DSSISKQALSEIETRHSEII
KLENRLRELHDMFMDMAMLV
ESQGEMI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 605 |
| 15N chemical shifts | 151 |
| 1H chemical shifts | 811 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | sx23 | 1 |
Entities:
Entity 1, sx23 207 residues - Formula weight is not available
| 1 | GLY | SER | ARG | ASP | ARG | PHE | MET | ASP | GLU | PHE | ||||
| 2 | PHE | GLU | GLN | VAL | GLU | GLU | ILE | ARG | GLY | PHE | ||||
| 3 | ILE | ASP | LYS | ILE | ALA | GLU | ASN | VAL | GLU | GLU | ||||
| 4 | VAL | LYS | ARG | LYS | HIS | SER | ALA | ILE | LEU | ALA | ||||
| 5 | SER | PRO | ASN | PRO | ASP | GLU | LYS | THR | LYS | GLU | ||||
| 6 | GLU | LEU | GLU | GLU | LEU | MET | SER | ASP | ILE | LYS | ||||
| 7 | LYS | THR | ALA | ASN | LYS | VAL | ARG | SER | LYS | LEU | ||||
| 8 | LYS | SER | ILE | GLU | GLN | SER | ILE | GLU | GLN | GLU | ||||
| 9 | GLU | GLY | LEU | ASN | ARG | SER | SER | ALA | ASP | LEU | ||||
| 10 | ARG | ILE | ARG | LYS | THR | GLN | HIS | SER | THR | LEU | ||||
| 11 | SER | ARG | LYS | PHE | VAL | GLU | VAL | MET | SER | GLU | ||||
| 12 | TYR | ASN | ALA | THR | GLN | SER | ASP | TYR | ARG | GLU | ||||
| 13 | ARG | CYS | LYS | GLY | ARG | ILE | GLN | ARG | GLN | LEU | ||||
| 14 | GLU | ILE | THR | GLY | ARG | THR | THR | THR | SER | GLU | ||||
| 15 | GLU | LEU | GLU | ASP | MET | LEU | GLU | SER | GLY | ASN | ||||
| 16 | PRO | ALA | ILE | PHE | ALA | SER | GLY | ILE | ILE | MET | ||||
| 17 | ASP | SER | SER | ILE | SER | LYS | GLN | ALA | LEU | SER | ||||
| 18 | GLU | ILE | GLU | THR | ARG | HIS | SER | GLU | ILE | ILE | ||||
| 19 | LYS | LEU | GLU | ASN | ARG | LEU | ARG | GLU | LEU | HIS | ||||
| 20 | ASP | MET | PHE | MET | ASP | MET | ALA | MET | LEU | VAL | ||||
| 21 | GLU | SER | GLN | GLY | GLU | MET | ILE |
Samples:
sample_1: sx23, [U-99% 13C; U-99% 15N], 700 uM; sodium phosphate 20 mM
sample_2: sx23, [U-99% 15N], 700 uM; sodium phosphate 20 mM
sample_3: sx23, [U-13C; U-15N; U-2H], 700 uM; sodium phosphate 20 mM
sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_3 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_3 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_3 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_3 | isotropic | sample_conditions_1 |
Software:
VNMR, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 4198 |
| PDB | |
| DBJ | BAA01231 BAA02089 BAA07151 BAA28865 BAC78519 |
| EMBL | CAG33299 CAH93304 |
| GB | AAA20940 AAA42195 AAA53519 AAB22525 AAB65500 |
| PIR | G01485 |
| PRF | 2116295A |
| REF | NP_001028037 NP_001126944 NP_001159375 NP_004594 NP_058081 |
| SP | O35526 P32850 P32851 Q16623 Q5R4L2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts