BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15669

Title: Sequence-specific 1H, 13C and 15N resonance assignments of the GRP1 PH domain   PubMed: 19636878

Deposition date: 2008-02-21 Original release date: 2008-05-30

Authors: He, Ju; Kutateladze, Tatiana

Citation: He, Ju; Kutateladze, Tatiana. "Sequence-specific 1H, 13C, and 15N resonance assignments of the GRP1 PH domain"  Biomol. NMR Assignments 2, 97-99 (2008).

Assembly members:
GRP1, polymer, 125 residues, 15373.4 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
GRP1: TFFNPDREGWLLKLGGRVKT WKRRWFILTDNCLYYFEYTT DKEPRGIIPLENLSIREVED PRKPNCFELYNPSHKGQVIK ACKTEADGRVVEGNHVVYRI SAPSPEEKEEWMKSIKASIS RDPFY

Data sets:
Data typeCount
13C chemical shifts475
15N chemical shifts124
1H chemical shifts882

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PH domain of GRP11

Entities:

Entity 1, PH domain of GRP1 125 residues - 15373.4 Da.

1   THRPHEPHEASNPROASPARGGLUGLYTRP
2   LEULEULYSLEUGLYGLYARGVALLYSTHR
3   TRPLYSARGARGTRPPHEILELEUTHRASP
4   ASNCYSLEUTYRTYRPHEGLUTYRTHRTHR
5   ASPLYSGLUPROARGGLYILEILEPROLEU
6   GLUASNLEUSERILEARGGLUVALGLUASP
7   PROARGLYSPROASNCYSPHEGLULEUTYR
8   ASNPROSERHISLYSGLYGLNVALILELYS
9   ALACYSLYSTHRGLUALAASPGLYARGVAL
10   VALGLUGLYASNHISVALVALTYRARGILE
11   SERALAPROSERPROGLUGLULYSGLUGLU
12   TRPMETLYSSERILELYSALASERILESER
13   ARGASPPROPHETYR

Samples:

sample_1: GRP1, [U-15N], 1 ± 0.1 mM; NaCl 300 ± 0.1 mM

sample_2: GRP1, [U-13C; U-15N], 1 ± 0.1 mM; NaCl 300 ± 0.1 mM

sample_conditions_1: ionic strength: 0.3 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA33433 BAC29342 BAD90330 BAE25057 BAE34915
EMBL CAA06434 CAA11686
GB AAB41445 AAB60876 AAF23858 AAH07189 AAH08191
REF NP_001072881 NP_001157020 NP_001238790 NP_004218 NP_035312
SP O08967 O43739 P97696
TPG DAA15150

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts