BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 1567

Title: Evidence for Two Interconverting Protein Isomers in the Methotrexate Complex of Dihydrofolate Reductase from Escherichia coli

Deposition date: 1995-07-31 Original release date: 1995-07-31

Authors: Falzone, Christopher; Wright, Peter; Benkovic, Stephen

Citation: Falzone, Christopher; Wright, Peter; Benkovic, Stephen. "Evidence for Two Interconverting Protein Isomers in the Methotrexate Complex of Dihydrofolate Reductase from Escherichia coli"  Biochemistry 30, 2184-2191 (1991).

Assembly members:
dihydrofolate reductase, polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: not available

Entity Sequences (FASTA):
dihydrofolate reductase: XXSLIAALAVXXXXXXXXXX XWXLXXXLXWFXXXTXXXXX IXXXHXWXXXXXXLXXXXXX ILXXXXXXXXXVTWVVXVXX AIXXXXXXXXXXVIXXXXXY XXFXXXAXXLYLTHIXXXXX XXXHFXXYXXXXWXXXFXXX HXXXXXXXHXXXFXIL

Data sets:
Data typeCount
1H chemical shifts240

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dihydrofolate reductase1

Entities:

Entity 1, dihydrofolate reductase 156 residues - Formula weight is not available

1   XXSERLEUILEALAALALEUALAVAL
2   XXXXXXXXXX
3   XTRPXLEUXXXLEUXTRP
4   PHEXXXTHRXXXXX
5   ILEXXXHISXTRPXXX
6   XXXLEUXXXXXX
7   ILELEUXXXXXXXX
8   XVALTHRTRPVALVALXVALXX
9   ALAILEXXXXXXXX
10   XXVALILEXXXXXTYR
11   XXPHEXXXALAXXLEU
12   TYRLEUTHRHISILEXXXXX
13   XXXHISPHEXXTYRXX
14   XXTRPXXXPHEXXX
15   HISXXXXXXXHISX
16   XXPHEXILELEU

Samples:

sample_one:

sample_condition_set_one: pH: 6.83 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 1230 1566