BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15682

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Epidermal Growth Factor-like 2 domain from Microneme Protein 6 (Toxoplasma gondii)   PubMed: 19636901

Deposition date: 2008-03-14 Original release date: 2008-10-03

Authors: Sawmynaden, Kovilen; Saouros, Savvas; Marchant, Jan; Simpson, Peter; Matthews, Stephen

Citation: Sawmynaden, Kovilen; Saouros, Savvas; Marchant, Jan; Simpson, Peter; Matthews, Stephen. "Complete NMR assignments for the second EGF domain of MIC6 from Toxoplasma gondii and re-assignment in complex with the galectin-like domain of MIC1"  Biomol. NMR Assignments 2, 187-189 (2008).

Assembly members:
Epidermal_Growth_Factor-like_domain_2, polymer, 61 residues, 6362 Da.

Natural source:   Common Name: Toxoplasma gondii   Taxonomy ID: 5811   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Toxoplasma gondii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Epidermal_Growth_Factor-like_domain_2: MANFVQLSETPAACSSNPCG PEAAGTCKETNSGYICRCNQ GYRISLDGTGNVTCIVRQES G

Data sets:
Data typeCount
13C chemical shifts218
15N chemical shifts54
1H chemical shifts332

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Epidermal Growth Factor-like domain 21

Entities:

Entity 1, Epidermal Growth Factor-like domain 2 61 residues - 6362 Da.

1   METALAASNPHEVALGLNLEUSERGLUTHR
2   PROALAALACYSSERSERASNPROCYSGLY
3   PROGLUALAALAGLYTHRCYSLYSGLUTHR
4   ASNSERGLYTYRILECYSARGCYSASNGLN
5   GLYTYRARGILESERLEUASPGLYTHRGLY
6   ASNVALTHRCYSILEVALARGGLNGLUSER
7   GLY

Samples:

sample_1: Epidermal Growth Factor-like domain 2, [U-98% 13C; U-98% 15N], 0.5 mM; potassium phosphate 25 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HC(CO)NH-TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - Peak picking, Viewing NMR spectra

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 15685
PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts