BMRB Entry 15752
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15752
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Title: Structure of a Glycosylphosphatidylinositol-anchored Domain from a Trypanosome Variant Surface Glycoprotein PubMed: 18003615
Deposition date: 2008-05-05 Original release date: 2008-11-12
Authors: Jones, Nicola; Nietlispach, Daniel; Sharma, Reuben; Burke, David; Eyres, Isobel; Mues, Marsilius; Mott, Helen; Carrington, Mark
Citation: Jones, Nicola; Nietlispach, Daniel; Sharma, Reuben; Burke, David; Eyres, Isobel; Mues, Marsilius; Mott, Helen; Carrington, Mark. "Structure of a glycosylphosphatidylinositol-anchored domain from a trypanosome variant surface glycoprotein" J. Biol. Chem. 283, 3584-3593 (2008).
Assembly members:
Variant_Surface_Glycoprotein_ILTat1.24, polymer, 48 residues, 5141.820 Da.
Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 5691 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma brucei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Variant_Surface_Glycoprotein_ILTat1.24: GTKASKSGVPVTQTQTAGAD
TTAEKCKGKGEKDCKSPDCK
WEGGTCKD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 276 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 48 residues - 5141.820 Da.
Residue G1 represents a residual non-native residue from affinity tag cleavage. This is the second C-terminal subdomain of the Variant Surface Glycoprotein ILTat1.24
| 1 | GLY | THR | LYS | ALA | SER | LYS | SER | GLY | VAL | PRO | ||||
| 2 | VAL | THR | GLN | THR | GLN | THR | ALA | GLY | ALA | ASP | ||||
| 3 | THR | THR | ALA | GLU | LYS | CYS | LYS | GLY | LYS | GLY | ||||
| 4 | GLU | LYS | ASP | CYS | LYS | SER | PRO | ASP | CYS | LYS | ||||
| 5 | TRP | GLU | GLY | GLY | THR | CYS | LYS | ASP |
Samples:
sample_1: ILTat1.24 C2-domain 0.5 mM; sodium phosphate 50 mM; sodium chloride 150 mM; sodium azide 0.05%; D2O 10%; H2O 90%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
AZARA, Boucher - processing
ANSIG, Kraulis - data analysis
ARIA, Linge, O, . - structure solution
NMR spectrometers:
- Bruker DRX 500 MHz