BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 1582

Title: Deletion of the omega-loop in the Active Site of Staphylococcal Nuclease 2. Effects on Protein Structure and Dynamics

Deposition date: 1995-07-31 Original release date: 1995-07-31

Authors: Baldisseri, Donna; Torchia, Dennis; Poole, Leslie; Gerlt, John

Citation: Baldisseri, Donna; Torchia, Dennis; Poole, Leslie; Gerlt, John. "Deletion of the omega-loop in the Active Site of Staphylococcal Nuclease 2. Effects on Protein Structure and Dynamics"  Biochemistry 30, 3628-3633 (1991).

Assembly members:
micrococcal nuclease, polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 1280   Superkingdom: bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: not available   Host organism: Escherichia coli

Entity Sequences (FASTA):
micrococcal nuclease: XXXXXXXXXXXXKLHKEPAT LIKAIDGDTVKLMYKGQPMT FRLLLVDTPDTKHPKKGVEK YGPEASAFTKKMVENAKKIE VEFNKGQRTDKYGRGLAYIY ADGKMVNEALVRQGLAKVAY VYKPNNTHEQHLRKSEAQAK KEKLNIWSENDADSGQ

Data sets:
Data typeCount
15N chemical shifts129
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1micrococcal nuclease1

Entities:

Entity 1, micrococcal nuclease 156 residues - Formula weight is not available

1   XXXXXXXXXX
2   XXLYSLEUHISLYSGLUPROALATHR
3   LEUILELYSALAILEASPGLYASPTHRVAL
4   LYSLEUMETTYRLYSGLYGLNPROMETTHR
5   PHEARGLEULEULEUVALASPTHRPROASP
6   THRLYSHISPROLYSLYSGLYVALGLULYS
7   TYRGLYPROGLUALASERALAPHETHRLYS
8   LYSMETVALGLUASNALALYSLYSILEGLU
9   VALGLUPHEASNLYSGLYGLNARGTHRASP
10   LYSTYRGLYARGGLYLEUALATYRILETYR
11   ALAASPGLYLYSMETVALASNGLUALALEU
12   VALARGGLNGLYLEUALALYSVALALATYR
13   VALTYRLYSPROASNASNTHRHISGLUGLN
14   HISLEUARGLYSSERGLUALAGLNALALYS
15   LYSGLULYSLEUASNILETRPSERGLUASN
16   ASPALAASPSERGLYGLN

Samples:

sample_one:

sample_condition_set_one: pH: 6.4 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 136 1581 16585 1704 1874 1875 1876 1877 1878 188 189 2784 2785
PDB
DBJ BAT21500
EMBL CAA24594
GB AAC14660 ABF58092 ABF58093 ABF58094 ABF58095
PRF 1109959A 710414A
REF WP_049881958 WP_052471469
SP P00644

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