BMRB Entry 15831

Name: chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y2-receptor
Published: 2009-02-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15831

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y2-receptor PubMed: 19125388

Deposition date: 2008-06-27 Original release date: 2009-02-13

Authors: Zerbe, Oliver; Walser, Reto; Zou, Chao; Kumaran, Sowmini

Citation: Zou, Chao; Kumaran, Sowmini; Walser, Reto; Zerbe, Oliver. "Properties of the N-terminal domains from Y receptors probed by NMR spectroscopy." J. Pept. Sci. 15, 184-191 (2009).

Assembly members:
N-Y2, polymer, 50 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
N-Y2: MGPIGAEADENQTVEEMKVE QYGPQTTPRGELVPDPEPEL IDSTKLIEVQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
heteronucl_NOEs
- heteronuclear_noe_list_1

Data type	Count
15N chemical shifts	43
1H chemical shifts	43
heteronuclear NOE values	43

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-Y2	1

Entities:

Entity 1, N-Y2 50 residues - Formula weight is not available

1	MET	GLY	PRO	ILE	GLY	ALA	GLU	ALA	ASP	GLU
2	ASN	GLN	THR	VAL	GLU	GLU	MET	LYS	VAL	GLU
3	GLN	TYR	GLY	PRO	GLN	THR	THR	PRO	ARG	GLY
4	GLU	LEU	VAL	PRO	ASP	PRO	GLU	PRO	GLU	LEU
5	ILE	ASP	SER	THR	LYS	LEU	ILE	GLU	VAL	GLN

Samples:

sample_1: N-Y2, [U-15N], 0.2 mM; MES 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: N-Y2, [U-15N], 0.4 mM; MES 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N NOE	sample_2	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 700 MHz

DBJ	BAG74149
GB	AAA93170 AAB04120 AAB07760 AAC50281 AAC51115
REF	NP_000901 NP_001012655 NP_001028004 XP_002832879 XP_003257918
SP	P49146 Q5IS62 Q9GK74

Biological Magnetic Resonance Data Bank