BMRB Entry 15832
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15832
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Title: chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y5-receptor PubMed: 19125388
Deposition date: 2008-06-27 Original release date: 2009-02-13
Authors: Zerbe, Oliver; Walser, Reto; Zou, Chao; Kumaran, Sowmini
Citation: Zou, Chao; Kumaran, Sowmini; Walser, Reto; Zerbe, Oliver. "Properties of the N-terminal domains from Y receptors probed by NMR spectroscopy." J. Pept. Sci. 15, 184-191 (2009).
Assembly members:
N-Y5, polymer, 51 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
N-Y5: MSFYSKQDYNMDLELDEYYN
KTLATENNTAATRNSDFPVW
DDYKSSVDDLQ
- assigned_chemical_shifts
- heteronucl_NOEs
Data type | Count |
15N chemical shifts | 48 |
1H chemical shifts | 48 |
heteronuclear NOE values | 47 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | N-Y5 | 1 |
Entities:
Entity 1, N-Y5 51 residues - Formula weight is not available
1 | MET | SER | PHE | TYR | SER | LYS | GLN | ASP | TYR | ASN | ||||
2 | MET | ASP | LEU | GLU | LEU | ASP | GLU | TYR | TYR | ASN | ||||
3 | LYS | THR | LEU | ALA | THR | GLU | ASN | ASN | THR | ALA | ||||
4 | ALA | THR | ARG | ASN | SER | ASP | PHE | PRO | VAL | TRP | ||||
5 | ASP | ASP | TYR | LYS | SER | SER | VAL | ASP | ASP | LEU | ||||
6 | GLN |
Samples:
sample_1: N-Y5, [U-15N], 0.5 mM; sodium phosphate 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_2: N-Y5, [U-15N], 0.5 mM; sodium phosphate 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 310 K
sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N NOE | sample_2 | isotropic | sample_conditions_2 |
Software:
XEASY, Bartels et al. - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz
Related Database Links:
DBJ | BAG74150 BAK62588 |
GB | AAC50623 AAC50741 AAC51295 AAG40773 AAH42416 |
REF | NP_001028005 NP_001266807 NP_006165 XP_003899379 XP_004040628 |
SP | Q15761 |
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