BMRB Entry 15855

Assembly:

Entities:

Entity 1, Gadd45a 168 residues - 18627 Da.

Residues from -2 to 0 (GPH) represent a non-native tag in N-terminus

Samples:

HGa_CN: Gadd45a, [U-99% 13C; U-99% 15N], 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_N: Gadd45a, [U-99% 15N], 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_D2O: Gadd45a 195 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT-d10 2 mM; EDTA-d12 2 mM; sodium azide 0.03%

HGa_15NLeu: Gadd45a, [U-15N]-Leu, 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_15NVal: Gadd45a, [U-15N]-Val, 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_15NIle: Gadd45a, [U-15N]-Ile, 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

HGa_15NPhe_Tyr: Gadd45a, [U-15N]-Phe/Tyr, 450 uM; sodium phosphate 20 mM; potassium chloride 100 mM; DTT 2 mM; EDTA 2 mM; sodium azide 0.03%

sample_conditions_1: ionic strength: 0.27 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

xwinnmr v3.5, Bruker Biospin - collection

NMRPipe v1.02, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

• Bruker Avance 600 MHz
• Bruker Avance II 800 MHz
• Bruker Avance II 900 MHz