BMRB Entry 15882
BMRB Entry DOI: doi:10.13018/BMR15882
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Title: 1H, 15N, 13C backbone assignment of the SPF45 UHM domain PubMed: 17589525
Deposition date: 2008-07-22 Original release date: 2008-10-01
Authors: Corsini, Lorenzo; Sattler, Michael
Citation: Corsini, Lorenzo; Bonnal, Sophie; Basquin, Jerome; Hothorn, Michael; Scheffzek, Klaus; Valcarcel, Juan; Sattler, Michael. "U2AF-homology motif interactions are required for alternative splicing regulation by SPF45." Nat. Struct. Biol. 14, 620-629 (2007).
Assembly members:
SPF45, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SPF45: GAMKCPTKVVLLRNMVGAGE
VDEDLEVETKEECEKYGKVG
KCVIFEIPGAPDDEAVRIFL
EFERVESAIKAVVDLNGRYF
GGRVVKACFYNLDKFRVLDL
AEQV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 196 |
| 1H chemical shifts | 96 |
| 15N chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SPF45 | 1 |
Entities:
Entity 1, SPF45 104 residues - Formula weight is not available
| 1 | GLY | ALA | MET | LYS | CYS | PRO | THR | LYS | VAL | VAL | ||||
| 2 | LEU | LEU | ARG | ASN | MET | VAL | GLY | ALA | GLY | GLU | ||||
| 3 | VAL | ASP | GLU | ASP | LEU | GLU | VAL | GLU | THR | LYS | ||||
| 4 | GLU | GLU | CYS | GLU | LYS | TYR | GLY | LYS | VAL | GLY | ||||
| 5 | LYS | CYS | VAL | ILE | PHE | GLU | ILE | PRO | GLY | ALA | ||||
| 6 | PRO | ASP | ASP | GLU | ALA | VAL | ARG | ILE | PHE | LEU | ||||
| 7 | GLU | PHE | GLU | ARG | VAL | GLU | SER | ALA | ILE | LYS | ||||
| 8 | ALA | VAL | VAL | ASP | LEU | ASN | GLY | ARG | TYR | PHE | ||||
| 9 | GLY | GLY | ARG | VAL | VAL | LYS | ALA | CYS | PHE | TYR | ||||
| 10 | ASN | LEU | ASP | LYS | PHE | ARG | VAL | LEU | ASP | LEU | ||||
| 11 | ALA | GLU | GLN | VAL |
Samples:
sample_1: SPF45, [U-100% 13C; U-100% 15N], 0.5 ± 0.1 mM
sample_conditions_1: temperature: 300 K; pH: 7.00; pressure: 1 atm; ionic strength: 0.2 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView v5.0.2, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAE91606 BAG37457 BAI47074 BAK63294 |
| GB | AAC64085 AAH07871 AAH09064 AAH34896 AAH39322 |
| REF | NP_001013076 NP_001094553 NP_001139019 NP_001244661 NP_001267436 |
| SP | Q8JZX4 Q96I25 |
| TPG | DAA16000 DAA23631 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts