BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15884

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for CTL9 native state at pH 3.8   PubMed: 18707127

Deposition date: 2008-07-24 Original release date: 2008-09-19

Authors: Shan, Bing; Raleigh, Daniel

Citation: Shan, Bing; Raleigh, Daniel. "The Low pH Unfolded State of the C-terminal Domain of the Ribosomal Protein L9 Contains Significant Secondary Structure in the Absence of Denaturant but is No More Compact than the Low pH Urea Unfolded State"  Biochemistry 47, 9565-9573 (2008).

Assembly members:
CTL9, polymer, 92 residues, Formula weight is not available

Natural source:   Common Name: Bacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CTL9: AAEELANAKKLKEQLEKLTV TIPAKAGEGGRLFGSITSKQ IAESLQAQHGLKLDKRKIEL ADAIRALGYTNVPVKLHPEV TATLKVHVTEQK

Data sets:
Data typeCount
13C chemical shifts257
15N chemical shifts82
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CTL91

Entities:

Entity 1, CTL9 92 residues - Formula weight is not available

CTL9 is the c terminal domain of protein L9, consisting of residues 58-149

1   ALAALAGLUGLULEUALAASNALALYSLYS
2   LEULYSGLUGLNLEUGLULYSLEUTHRVAL
3   THRILEPROALALYSALAGLYGLUGLYGLY
4   ARGLEUPHEGLYSERILETHRSERLYSGLN
5   ILEALAGLUSERLEUGLNALAGLNHISGLY
6   LEULYSLEUASPLYSARGLYSILEGLULEU
7   ALAASPALAILEARGALALEUGLYTYRTHR
8   ASNVALPROVALLYSLEUHISPROGLUVAL
9   THRALATHRLEULYSVALHISVALTHRGLU
10   GLNLYS

Samples:

sample_1: CTL9, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%; sodium acetate 20 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 120 mM; pH: 3.8; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
HNCACOsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15883 17460
PDB
EMBL CAA43972
GB AAA22701 AKM20686 AKU26730 KFL16938 KFX34457
PRF 0701226A 1714237A
REF WP_033008865 WP_050368025
SP P02417

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts