BMRB Entry 15885

Name: Backbone assignment of the UHM domain of Puf60 in the free form
Published: 2008-10-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15885

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone assignment of the UHM domain of Puf60 in the free form

Deposition date: 2008-07-25 Original release date: 2008-10-15

Authors: Corsini, Lorenzo; Sattler, Michael

Citation: Corsini, Lorenzo; Sattler, Michael. "Backbone assignment of the UHM domain of Puf60 free and bound to five ligands" Biomol. NMR Assignments 2, 211-214 (2008).

Assembly members:
UHM_domain_of_Puf60, polymer, 103 residues, 12637.1 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
UHM_domain_of_Puf60: GAMESTVMVLRNMVDPKDID DDLEGEVTEECGKFGAVNRV IIYQEKQGEEEDAEIIVKIF VEFSIASETHKAIQALNGRW FAGRKVVAEVYDQERFDNSD LSA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	197
15N chemical shifts	99
1H chemical shifts	99

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UHM domain of Puf60	1

Entities:

Entity 1, UHM domain of Puf60 103 residues - 12637.1 Da.

1

GLY

ALA

MET

GLU

SER

THR

VAL

MET

VAL

LEU

2

ARG

ASN

MET

VAL

ASP

PRO

LYS

ASP

ILE

ASP

3

ASP

LEU

GLU

GLY

GLU

VAL

THR

GLU

4

CYS

GLY

LYS

PHE

GLY

ALA

VAL

ASN

ARG

VAL

5

ILE

TYR

GLN

GLU

LYS

GLN

GLY

GLU

6

GLU

ASP

ALA

GLU

ILE

VAL

LYS

ILE

PHE

7

VAL

GLU

PHE

SER

ILE

ALA

SER

GLU

THR

HIS

8

LYS

ALA

ILE

GLN

ALA

LEU

ASN

GLY

ARG

TRP

9

PHE

ALA

GLY

ARG

LYS

VAL

ALA

GLU

VAL

10

TYR

ASP

GLN

GLU

ARG

PHE

ASP

ASN

SER

ASP

11

LEU

SER

ALA

Samples:

sample_1: UHM domain of Puf60, [U-100% 13C; U-100% 15N], 0.4 ± 0.1 mM; NaH2PO4 50 mM; NaCl 150 mM; b-mercaptoethanol 5 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz

BMRB	15887 15888 15889 15890 15891
PDB	2DNY 3DXB 3UE2 3US5
DBJ	BAE28998 BAE30474 BAE40622 BAF85062 BAI46053
EMBL	CAG33424 CAH89379 CAH93447
GB	AAB41656 AAD44358 AAF05605 AAF23589 AAF27522
REF	NP_001039598 NP_001127017 NP_001128734 NP_001129505 NP_001158072
SP	Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25
TPG	DAA22863

Biological Magnetic Resonance Data Bank