BMRB Entry 15889

Name: 1H, 15N chemical shifts of the UHM domain of Puf60 in complex with SF3b155(194-229)
Published: 2008-10-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15889

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 15N chemical shifts of the UHM domain of Puf60 in complex with SF3b155(194-229) PubMed: 19636907

Deposition date: 2008-07-25 Original release date: 2008-10-15

Authors: Corsini, Lorenzo; Sattler, Michael

Citation: Corsini, Lorenzo; Sattler, Michael. "Backbone assignment of the UHM domain of Puf60 free and bound to five ligands" Biomol. NMR Assignments 2, 211-214 (2008).

Assembly members:
UHM_domain_of_Puf60, polymer, 110 residues, 12637.1 Da.
SF3b155(194-229), polymer, 40 residues, 4366.7 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
UHM_domain_of_Puf60: MKHHHHHHPMESTVMVLRNM VDPKDIDDDLEGEVTEECGK FGAVNRVIIYQEKQGEEEDA EIIVKIFVEFSIASETHKAI QALNGRWFAGRKVVAEVYDQ ERFDNSDLSA
SF3b155(194-229): GAMGSKRKRRWDQTADQTPG ATPKKLSSWDQAETPGHTPS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UHM domain of Puf60	1
2	SF3b155(194-229)	2

Entities:

Entity 1, UHM domain of Puf60 110 residues - 12637.1 Da.

1	MET	LYS	HIS	HIS	HIS	HIS	HIS	HIS	PRO	MET
2	GLU	SER	THR	VAL	MET	VAL	LEU	ARG	ASN	MET
3	VAL	ASP	PRO	LYS	ASP	ILE	ASP	ASP	ASP	LEU
4	GLU	GLY	GLU	VAL	THR	GLU	GLU	CYS	GLY	LYS
5	PHE	GLY	ALA	VAL	ASN	ARG	VAL	ILE	ILE	TYR
6	GLN	GLU	LYS	GLN	GLY	GLU	GLU	GLU	ASP	ALA
7	GLU	ILE	ILE	VAL	LYS	ILE	PHE	VAL	GLU	PHE
8	SER	ILE	ALA	SER	GLU	THR	HIS	LYS	ALA	ILE
9	GLN	ALA	LEU	ASN	GLY	ARG	TRP	PHE	ALA	GLY
10	ARG	LYS	VAL	VAL	ALA	GLU	VAL	TYR	ASP	GLN
11	GLU	ARG	PHE	ASP	ASN	SER	ASP	LEU	SER	ALA

Entity 2, SF3b155(194-229) 40 residues - 4366.7 Da.

1	GLY	ALA	MET	GLY	SER	LYS	ARG	LYS	ARG	ARG
2	TRP	ASP	GLN	THR	ALA	ASP	GLN	THR	PRO	GLY
3	ALA	THR	PRO	LYS	LYS	LEU	SER	SER	TRP	ASP
4	GLN	ALA	GLU	THR	PRO	GLY	HIS	THR	PRO	SER

Samples:

minimal_complex_of_Puf60_and_SF3b155(194-229): UHM domain of Puf60, [U-100% 13C; U-100% 15N], 0.0914 ± 0.01 mM; SF3b155(194-229) 0.422 ± 0.01 mM; NaH2PO4 50 mM; NaCl 150 mM; b-mercaptoethanol 5 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 0.2 M; pH: 6.8; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	minimal_complex_of_Puf60_and_SF3b155(194-229)	isotropic	sample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker DRX 500 MHz

BMRB	15885 15887 15888 15890 15891
PDB	2DNY 3DXB
DBJ	BAE28998 BAE30474 BAE40622 BAF85062 BAI46053 BAB28369 BAB40140 BAE24537 BAE27592
EMBL	CAG33424 CAH89379 CAH93447
GB	AAB41656 AAD44358 AAF05605 AAF23589 AAF27522 AAC97189 AAG01404 AAH03419 AAH15530 AAH28959
REF	NP_001039598 NP_001127017 NP_001128734 NP_001129505 NP_001158072 NP_001179923 NP_001252892 NP_036565 NP_112456 NP_445878
SP	Q2HJG2 Q3UEB3 Q5R469 Q9UHX1 Q9WV25 O75533 Q99NB9
TPG	DAA22863 DAA32571

Biological Magnetic Resonance Data Bank