BMRB Entry 15961
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15961
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Title: Backbone and Side chain 1H, 13C, and 15N Chemical Shift Assignments for Microneme protein 2 Associated protein PubMed: 19636952
Deposition date: 2008-09-24 Original release date: 2009-03-03
Authors: Liu, Bing; Sawmynaden, Kovilen; Simpson, Pete; Matthews, Steve
Citation: Liu, Bing; Sawmynaden, Kovilen; Marchant, Jan; Simpson, Peter; Matthews, Stephen. "Complete resonance assignments for the MIC2 associated protein from Toxoplasma gondii" Biomol. NMR Assignments 3, 81-83 (2009).
Assembly members:
M2AP, polymer, 182 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
M2AP: TFLELVEVPCNSVHVQGVMT
PNQMVKVTGAGWDNGVLEFY
VTRPTKTGGDTSRSHLASIM
CYSKDIDGVPSDKAGKCFLK
NFSGEDSSEIDEKEVSLPIK
SHNDAFMFVCSSNDGSALQC
DVFALDNTNSSDGWKVNTVD
LGVSVSPDLAFGLTADGVKV
KKLYASSGLTAINDDPSLGC
KA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 735 |
| 15N chemical shifts | 180 |
| 1H chemical shifts | 1134 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | M2AP | 1 |
Entities:
Entity 1, M2AP 182 residues - Formula weight is not available
| 1 | THR | PHE | LEU | GLU | LEU | VAL | GLU | VAL | PRO | CYS | ||||
| 2 | ASN | SER | VAL | HIS | VAL | GLN | GLY | VAL | MET | THR | ||||
| 3 | PRO | ASN | GLN | MET | VAL | LYS | VAL | THR | GLY | ALA | ||||
| 4 | GLY | TRP | ASP | ASN | GLY | VAL | LEU | GLU | PHE | TYR | ||||
| 5 | VAL | THR | ARG | PRO | THR | LYS | THR | GLY | GLY | ASP | ||||
| 6 | THR | SER | ARG | SER | HIS | LEU | ALA | SER | ILE | MET | ||||
| 7 | CYS | TYR | SER | LYS | ASP | ILE | ASP | GLY | VAL | PRO | ||||
| 8 | SER | ASP | LYS | ALA | GLY | LYS | CYS | PHE | LEU | LYS | ||||
| 9 | ASN | PHE | SER | GLY | GLU | ASP | SER | SER | GLU | ILE | ||||
| 10 | ASP | GLU | LYS | GLU | VAL | SER | LEU | PRO | ILE | LYS | ||||
| 11 | SER | HIS | ASN | ASP | ALA | PHE | MET | PHE | VAL | CYS | ||||
| 12 | SER | SER | ASN | ASP | GLY | SER | ALA | LEU | GLN | CYS | ||||
| 13 | ASP | VAL | PHE | ALA | LEU | ASP | ASN | THR | ASN | SER | ||||
| 14 | SER | ASP | GLY | TRP | LYS | VAL | ASN | THR | VAL | ASP | ||||
| 15 | LEU | GLY | VAL | SER | VAL | SER | PRO | ASP | LEU | ALA | ||||
| 16 | PHE | GLY | LEU | THR | ALA | ASP | GLY | VAL | LYS | VAL | ||||
| 17 | LYS | LYS | LEU | TYR | ALA | SER | SER | GLY | LEU | THR | ||||
| 18 | ALA | ILE | ASN | ASP | ASP | PRO | SER | LEU | GLY | CYS | ||||
| 19 | LYS | ALA |
Samples:
sample_1: M2AP, [U-100% 13C; U-100% 15N], 0.5 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 5.9; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| GB | AAK51546 EPR60220 EPT26499 ESS31155 KFG36006 |
| REF | XP_002370815 |
| TPE | CEL77075 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts