BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15967

Title: Structural characterization of IscU and its interaction with HscB   PubMed: 19492851

Deposition date: 2008-09-28 Original release date: 2008-09-28

Authors: Kim, Jin Hae; Fuzery, Anna; Marco, Tonelli; William, Westler; Markley, John

Citation: Kim, Jin Hae; Fuzery, Anna; Tonelli, Marco; Ta, Dennis; Westler, William; Vickery, Larry; Markley, John. "Structure and Dynamics of the Iron-Sulfur Cluster Assembly Scaffold Protein IscU and Its Interaction with the Cochaperone HscB."  Biochemistry 48, 6062-6071 (2009).

Assembly members:
IscU(D39A), polymer, 128 residues, Formula weight is not available
HscB, polymer, 171 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IscU(D39A): MAYSEKVIDHYENPRNVGSF DNNDENVGSGMVGAPACGAV MKLQIKVNDEGIIEDARFKT YGCGSAIASSSLVTEWVKGK SLDEAQAIKNTDIAEELELP PVKIHCSILAEDAIKAAIAD YKSKREAK
HscB: MDYFTLFGLPARYQLDTQAL SLRFQDLQRQYHPDKFASGS QAEQLAAVQQSATINQAWQT LRHPLMRAEYLLSLHGFDLA SEQHTVRDTAFLMEQLELRE ELDEIEQAKDEARLESFIKR VKKMFDTRHQLMVEQLDNET WDAAADTVRKLRFLDKLRSS AEQLEEKLLDF

Data sets:
Data typeCount
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IscU(D39A)1
2HscB2

Entities:

Entity 1, IscU(D39A) 128 residues - Formula weight is not available

1   METALATYRSERGLULYSVALILEASPHIS
2   TYRGLUASNPROARGASNVALGLYSERPHE
3   ASPASNASNASPGLUASNVALGLYSERGLY
4   METVALGLYALAPROALACYSGLYALAVAL
5   METLYSLEUGLNILELYSVALASNASPGLU
6   GLYILEILEGLUASPALAARGPHELYSTHR
7   TYRGLYCYSGLYSERALAILEALASERSER
8   SERLEUVALTHRGLUTRPVALLYSGLYLYS
9   SERLEUASPGLUALAGLNALAILELYSASN
10   THRASPILEALAGLUGLULEUGLULEUPRO
11   PROVALLYSILEHISCYSSERILELEUALA
12   GLUASPALAILELYSALAALAILEALAASP
13   TYRLYSSERLYSARGGLUALALYS

Entity 2, HscB 171 residues - Formula weight is not available

1   METASPTYRPHETHRLEUPHEGLYLEUPRO
2   ALAARGTYRGLNLEUASPTHRGLNALALEU
3   SERLEUARGPHEGLNASPLEUGLNARGGLN
4   TYRHISPROASPLYSPHEALASERGLYSER
5   GLNALAGLUGLNLEUALAALAVALGLNGLN
6   SERALATHRILEASNGLNALATRPGLNTHR
7   LEUARGHISPROLEUMETARGALAGLUTYR
8   LEULEUSERLEUHISGLYPHEASPLEUALA
9   SERGLUGLNHISTHRVALARGASPTHRALA
10   PHELEUMETGLUGLNLEUGLULEUARGGLU
11   GLULEUASPGLUILEGLUGLNALALYSASP
12   GLUALAARGLEUGLUSERPHEILELYSARG
13   VALLYSLYSMETPHEASPTHRARGHISGLN
14   LEUMETVALGLUGLNLEUASPASNGLUTHR
15   TRPASPALAALAALAASPTHRVALARGLYS
16   LEUARGPHELEUASPLYSLEUARGSERSER
17   ALAGLUGLNLEUGLUGLULYSLEULEUASP
18   PHE

Samples:

sample_1: IscU(D39A), [U-15N], 0.7 mM; TRIS 20 mM; DTT 5-10 mM; EDTA 0.5 mM; sodium chloride 150 mM; DSS 0.7 mM; sodium azide 0.02%; HscB 1.4 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian Unity-Inova 600 MHz

Related Database Links:

BMRB 16245 16603 17282 17836 17837 17844 18359 18360 18361 18362 18381 18750 18754 15541
PDB
DBJ BAA16423 BAB36818 BAG78339 BAI26774 BAI31803 BAA16421 BAB36816 BAG78337 BAI26772 BAI31801
EMBL CAD02745 CAP76981 CAQ32902 CAQ88187 CAQ99420 CAP76979 CAQ32900 CAQ88189 CAQ99418 CAR03969
GB AAC75582 AAG57643 AAL21436 AAN44075 AAN81505 AAA18299 AAC75580 AAG57641 AAN44073 AAN81502
PIR AE0824 E85897
REF NP_311422 NP_417024 NP_457073 NP_461477 NP_708368 NP_289084 NP_311420 NP_417022 NP_708366 NP_754934
SP P0ACD4 P0ACD5 P0ACD6 P0ACD7 A1AE64 A7ZPX0 A8A333 B1IWD4 B1LNI2

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts