BMRB Entry 15972
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15972
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Title: 1H, 13C and 15N resonance assignments of the PDZ of MAST205 in complex with the C-terminal tail from the rabies virus glycoprotein PubMed: 19636944
Deposition date: 2008-10-03 Original release date: 2009-01-15
Authors: Wolff, Nicolas; Terrien, Elouan; Simenel, Catherine; Lafon, Monique; Delepierre, Muriel
Citation: Terrien, Elouan; Simenel, Catherine; Prehaud, Christophe; Buc, Henri; Delepierre, Muriel; Lafon, Monique; Wolff, Nicolas. "1H, 13C and 15N resonance assignments of the PDZ of microtubule-associated serine/threonine kinase 205 (MAST205) in complex with the C-terminal motif from the rabies virus glycoprotein" Biomol. NMR Assignments 3, 45-48 (2009).
Assembly members:
Mast205, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Mast205: GGSMRPPIIIHRAGKKYGFT
LRAIRVYMGDSDVYTVHHMV
WHVEDGGPASEAGLRQGDLI
THVNGEPVHGLVHTEVVELI
LKSGNKVAISTTPLEN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 402 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 645 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 96 residues - Formula weight is not available
| 1 | GLY | GLY | SER | MET | ARG | PRO | PRO | ILE | ILE | ILE | ||||
| 2 | HIS | ARG | ALA | GLY | LYS | LYS | TYR | GLY | PHE | THR | ||||
| 3 | LEU | ARG | ALA | ILE | ARG | VAL | TYR | MET | GLY | ASP | ||||
| 4 | SER | ASP | VAL | TYR | THR | VAL | HIS | HIS | MET | VAL | ||||
| 5 | TRP | HIS | VAL | GLU | ASP | GLY | GLY | PRO | ALA | SER | ||||
| 6 | GLU | ALA | GLY | LEU | ARG | GLN | GLY | ASP | LEU | ILE | ||||
| 7 | THR | HIS | VAL | ASN | GLY | GLU | PRO | VAL | HIS | GLY | ||||
| 8 | LEU | VAL | HIS | THR | GLU | VAL | VAL | GLU | LEU | ILE | ||||
| 9 | LEU | LYS | SER | GLY | ASN | LYS | VAL | ALA | ILE | SER | ||||
| 10 | THR | THR | PRO | LEU | GLU | ASN |
Samples:
sample_1: Tris; NaCl, [U-100% 13C; U-100% 15N], 800 uM; Mast205 800 uM; H2O 88%; D2O 12%
sample_conditions_1: ionic strength: 150 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 16967 |
| PDB | |
| DBJ | BAA34527 BAB40778 |
| EMBL | CAD38775 |
| GB | AAH65499 EAX06948 EAX06949 EFB27522 EHB01606 |
| REF | NP_055927 XP_001105315 XP_002810932 XP_004025774 XP_004285802 |
| SP | Q6P0Q8 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts