BMRB Entry 15973
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15973
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Title: HGF-binding peptide-10 PubMed: 18973760
Deposition date: 2008-10-05 Original release date: 2008-11-11
Authors: Skelton, Nicholas; Runyon, Steven
Citation: Tam, Eric; Runyon, Steven; Santell, Lydia; Quan, CLiff; Yao, Xiaoyi; Kirchhofer, Daniel; Skelton, Nicholas; Lazarus, Robert. "Noncompetitive inhibition of Hepatocyte Growth Factor Dependent Met signaling by a phage-derived peptide" J. Mol. Biol. 385, 79-90 (2009).
Assembly members:
HGF-binding peptide-10, polymer, 15 residues, 1812.109 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HGF-binding peptide-10: VNWVCFRDVGCDWVL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 56 |
| 1H chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HGF-binding peptide-10 | 1 |
Entities:
Entity 1, HGF-binding peptide-10 15 residues - 1812.109 Da.
| 1 | VAL | ASN | TRP | VAL | CYS | PHE | ARG | ASP | VAL | GLY | ||||
| 2 | CYS | ASP | TRP | VAL | LEU |
Samples:
sample_1: sodium chloride 25 mM; D2O, [U-99% 2H], 5500 mM; sodium azide 1 mM; 1-4 dioxane 1 mM; entity mM
sample_2: sodium chloride 25 mM; D2O, [U-99% 2H], 55000 mM; sodium azide 1 mM; entity mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY-35 | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMR spectrometers:
- Bruker DRX 600 MHz