BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15994

Title: 1H-15N Backbone Chemical shifts Of the C-terminal part of the Phosphoprotein (XD) of Measle Virus in complex with C-terminal Part of the Nucleoprotein (Ntail)   PubMed: 19275899

Deposition date: 2008-10-20 Original release date: 2009-04-03

Authors: Bernard, Cedric; Bourhis, Jean Marie; Gely, Stephane; Morelli, Xavier; Longhi, Sonia; Darbon, Herv

Citation: Bernard, Cedric; Gely, Stephane; Bourhis, Jean-Marie; Morelli, Xavier; Longhi, Sonia; Darbon, Herv. "Interaction between the C-terminal Domains Of N and P Proteins of measles virus investigated by NMR"  FEBS Lett. 583, 1084-1089 (2009).

Assembly members:
XD, polymer, 44 residues, Formula weight is not available

Natural source:   Common Name: Measles   Taxonomy ID: 11234   Superkingdom: Viruses   Kingdom: not available   Genus/species: Morbillivirus Measles virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
XD: VIRSIIKSSRLEEDRKRYLM TLLDDIKGANDLAKFHQMLV KIIM

Data sets:
Data typeCount
15N chemical shifts43
1H chemical shifts43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1XD1

Entities:

Entity 1, XD 44 residues - Formula weight is not available

1   VALILEARGSERILEILELYSSERSERARG
2   LEUGLUGLUASPARGLYSARGTYRLEUMET
3   THRLEULEUASPASPILELYSGLYALAASN
4   ASPLEUALALYSPHEHISGLNMETLEUVAL
5   LYSILEILEMET

Samples:

sample_1: XD, [U-100% 15N], 0.25-1.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQC-NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 15993 16000
PDB
DBJ BAA09955 BAA09962 BAA33868 BAA33874 BAA34978
EMBL CAA34578 CAA34585 CAA91364 CAQ15998 CAQ15999
GB AAA46434 AAA46435 AAA46437 AAA63284 AAA63285
REF NP_056919
SP P03422 P35974 Q9WMB4

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts