BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16049

Title: human brain-type fatty acid binding protein (hb-FABP bound to linoleic acid (LNA))   PubMed: 19636954

Deposition date: 2008-12-01 Original release date: 2009-03-11

Authors: Oeemig, Jesper; Jorgensen, Mathilde; Hansen, Mikka; Petersen, Evamaria; Duroux, Laurent; Wimmer, Reinhard

Citation: Oeemig, Jesper; Jorgensen, Mathilde; Hansen, Mikka; Petersen, Evamaria; Duroux, Laurent; Wimmer, Reinhard. "Backbone and sidechain 1H, 13C and 15N resonance assignments of the human brain-type fatty acid binding protein (FABP7) in its apo form and the holo forms binding to DHA, oleic acid, linoleic acid and elaidic acid"  Biomol. NMR Assignments 3, 89-93 (2009).

Assembly members:
hb-FABP, polymer, 131 residues, Formula weight is not available
EIC, non-polymer, 280.445 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hb-FABP: VEAFCATWKLTNSQNFDEYM KALGVGFATRQVGNVTKPTV IISQEGDKVVIRTLSTFKNT EISFQLGEEFDETTADDRNC KSVVSLDGDKLVHIQKWDGK ETNFVREIKDGKMVMTLTFG DVVAVRHYEKA

Data sets:
Data typeCount
13C chemical shifts388
15N chemical shifts141
1H chemical shifts863

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hb-FABP1
2LINOLEIC ACID2

Entities:

Entity 1, hb-FABP 131 residues - Formula weight is not available

1   VALGLUALAPHECYSALATHRTRPLYSLEU
2   THRASNSERGLNASNPHEASPGLUTYRMET
3   LYSALALEUGLYVALGLYPHEALATHRARG
4   GLNVALGLYASNVALTHRLYSPROTHRVAL
5   ILEILESERGLNGLUGLYASPLYSVALVAL
6   ILEARGTHRLEUSERTHRPHELYSASNTHR
7   GLUILESERPHEGLNLEUGLYGLUGLUPHE
8   ASPGLUTHRTHRALAASPASPARGASNCYS
9   LYSSERVALVALSERLEUASPGLYASPLYS
10   LEUVALHISILEGLNLYSTRPASPGLYLYS
11   GLUTHRASNPHEVALARGGLUILELYSASP
12   GLYLYSMETVALMETTHRLEUTHRPHEGLY
13   ASPVALVALALAVALARGHISTYRGLULYS
14   ALA

Entity 2, LINOLEIC ACID - C18 H32 O2 - 280.445 Da.

1   EIC

Samples:

sample_1: hb-FABP, [U-98% 13C; U-98% 15N], 0.85 mM; D2O 5%; H2O 95%; DSS 1 mM; TRIS 10 mM; Linoleic acid, [U-95% 13C], 7.2 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 7.4; pressure: 1 atm; temperature: 298.1 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CARA v1.5.5, CARA - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16042 16046 16047 16048
PDB
DBJ BAA23324 BAA23645 BAD92052 BAE90445 BAF82525
EMBL CAA05773 CAC21646 CAG33338
GB AAB87141 AAD00507 AAH12299 AAY17257 ABM82363
REF NP_001020400 NP_001244643 NP_001270567 NP_001437 XP_002746967
SP O15540

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts