BMRB Entry 16053
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PDB ID: 2kbt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16053
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Title: Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method PubMed: 19140010
Deposition date: 2008-12-07 Original release date: 2009-03-20
Authors: Kumeta, Hiroyuki; Kobashigawa, Yoshinori; Ogura, Kenji; Inagaki, Fuyuhiko
Citation: Kobashigawa, Yoshinori; Kumeta, Hiroyuki; Ogura, Kenji; Inagaki, Fuyuhiko. "Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method" J. Biomol. NMR 43, 145-150 (2009).
Assembly members:
entity, polymer, 141 residues, 8004.950 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: GPGTFGTAKARYDFCARDRS
ELSLKEGDIIKILNKKGQQG
WWRGEIYGRIGWFPSNYVEE
DYSEYLPETGGGSGSSMEYK
LILNGKTLKGETTTEAVDAA
TAEKVFKQYANDGVDGEWTY
DDATKTFTVTEHSLEHHHHH
H
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 305 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 483 |
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