BMRB Entry 16062
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16062
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Title: NMR solution structure of the pheromone En-1 of Euplotes nobilii at -1.5 C PubMed: 19621350
Deposition date: 2008-12-17 Original release date: 2009-07-23
Authors: Pedrini, Bill; Alimenti, Claudio; Vallesi, Adriana; Luporini, Pierangelo; Wuthrich, Kurt
Citation: Alimenti, Claudio; Vallesi, Adriana; Pedrini, Bill; Wuthrich, Kurt; Luporini, Pierangelo. "Molecular cold-adaptation: Comparative analysis of two homologous families of psychrophilic and mesophilic signal proteins of the protozoan ciliate, Euplotes" IUBMB Life 61, 838-845 (2009).
Assembly members:
En-1, polymer, 52 residues, 5630.097 Da.
Natural source: Common Name: Euplotes nobilii Taxonomy ID: 184062 Superkingdom: Eukaryota Kingdom: not available Genus/species: Euplotes nobilii
Experimental source: Production method: purified from the natural source Host organism: Euplotes nobilii
Entity Sequences (FASTA):
En-1: NPEDWFTPDTCAYGDSNTAW
TTCTTPGQTCYTCCSSCFDV
VGEQACQMSAQC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 139 |
| 1H chemical shifts | 300 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | En-1 | 1 |
Entities:
Entity 1, En-1 52 residues - 5630.097 Da.
| 1 | ASN | PRO | GLU | ASP | TRP | PHE | THR | PRO | ASP | THR | ||||
| 2 | CYS | ALA | TYR | GLY | ASP | SER | ASN | THR | ALA | TRP | ||||
| 3 | THR | THR | CYS | THR | THR | PRO | GLY | GLN | THR | CYS | ||||
| 4 | TYR | THR | CYS | CYS | SER | SER | CYS | PHE | ASP | VAL | ||||
| 5 | VAL | GLY | GLU | GLN | ALA | CYS | GLN | MET | SER | ALA | ||||
| 6 | GLN | CYS |
Samples:
sample_1: En-1 1.0 mM; sodium phosphate 20 mM
sample_conditions_1: pH: 6.0; temperature: 275.0 K
sample_conditions_2: pH: 6.0; temperature: 271.7 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
Software:
CARA v1.4.1, Keller and Wuthrich - chemical shift assignment
ATNOS v1.2, Herrmann, Guntert, Wuthrich - peak picking
CANDID, Herrmann, Guntert and Wuthrich - distance constraint calculation, peak assignment
CYANA v1, Guntert, Mumenthaler and Wuthrich - torsion angle dynamics simulated annealing
OPALp, Luginbuhl, Guntert, Billeter and Wuthrich - refinement
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz