BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16103

Title: 1H, 13C, and 15N chemical shift assignments for murine FAIM-CTD   PubMed: 19168072

Deposition date: 2009-01-01 Original release date: 2009-04-22

Authors: Hemond, Michael; Wagner, Gerhard

Citation: Hemond, Michael; Rothstein, Thomas; Wagner, Gerhard. "Fas Apoptosis Inhibitory Molecule contains a novel beta sandwich in contact with a partially ordered domain"  J. Mol. Biol. 386, 1024-1037 (2009).

Assembly members:
FAIM-CTD, polymer, 94 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
FAIM-CTD: GPLGSENRSKTTSTWVLRLD GEDLRVVLEKDTMDVWCNGQ KMETAGEFVDDGTETHFSVG NHDCYIKAVSSGKRKEGIIH TLIVDNREIPELTQ

Data sets:
Data typeCount
13C chemical shifts364
15N chemical shifts96
1H chemical shifts561
residual dipolar couplings67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FAIM-CTD monomer1

Entities:

Entity 1, FAIM-CTD monomer 94 residues - Formula weight is not available

Residues 1-5 are derived from a non-native protease site and polypeptide linker

1   GLYPROLEUGLYSERGLUASNARGSERLYS
2   THRTHRSERTHRTRPVALLEUARGLEUASP
3   GLYGLUASPLEUARGVALVALLEUGLULYS
4   ASPTHRMETASPVALTRPCYSASNGLYGLN
5   LYSMETGLUTHRALAGLYGLUPHEVALASP
6   ASPGLYTHRGLUTHRHISPHESERVALGLY
7   ASNHISASPCYSTYRILELYSALAVALSER
8   SERGLYLYSARGLYSGLUGLYILEILEHIS
9   THRLEUILEVALASPASNARGGLUILEPRO
10   GLULEUTHRGLN

Samples:

sample_1: FAIM-CTD, [U-99% 13C; U-99% 15N], 0.25 – 0.5 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_2: FAIM-CTD, [U-99% 13C; U-99% 15N], 0.25 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM; Pf1 filamentous phage 0.6% w/v

sample_3: FAIM-CTD, [U-99% 13C], 1.8 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_4: FAIM-CTD, [U-10% 13C], 0.5 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_5: FAIM-CTD, [U-99% 15N], 0.25 – 0.9 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_6: FAIM-CTD 1.4 mM; sodium chloride 10 mM; TRIS 10 mM; DTT 5 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.3; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2anisotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_5isotropicsample_conditions_1
3D 1H-15N TOCSYsample_5isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_6isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D CCH-COSYsample_3isotropicsample_conditions_1

Software:

NMRPipe v2.4 Rev 2006.095.11.35, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAB24225
GB AAD23879 AAH79662 EDL20994 EDL20995
REF NP_001116323 NP_035940
SP Q9WUD8

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts