BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16137

Title: NMR structure of the first SAM domain from AIDA1

Deposition date: 2009-01-27 Original release date: 2009-10-20

Authors: Donaldson, Logan

Citation: Donaldson, Logan. "NMR structure of the first SAM domain from AIDA1"  Not known ., .-..

Assembly members:
AIDA1_SAM1, polymer, 103 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
AIDA1_SAM1: GSDITSLYKKAGSEMNGPRC PVQTVGQWLESIGLPQYENH LMANGFDNVQFMGSNVMEDQ DLLEIGILNSGHRQRILQAI QLLPKMRPIGHDGYHPTSVA EWL

Data sets:
Data typeCount
13C chemical shifts257
15N chemical shifts61
1H chemical shifts411

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AIDA1_SAM11

Entities:

Entity 1, AIDA1_SAM1 103 residues - Formula weight is not available

1   GLYSERASPILETHRSERLEUTYRLYSLYS
2   ALAGLYSERGLUMETASNGLYPROARGCYS
3   PROVALGLNTHRVALGLYGLNTRPLEUGLU
4   SERILEGLYLEUPROGLNTYRGLUASNHIS
5   LEUMETALAASNGLYPHEASPASNVALGLN
6   PHEMETGLYSERASNVALMETGLUASPGLN
7   ASPLEULEUGLUILEGLYILELEUASNSER
8   GLYHISARGGLNARGILELEUGLNALAILE
9   GLNLEULEUPROLYSMETARGPROILEGLY
10   HISASPGLYTYRHISPROTHRSERVALALA
11   GLUTRPLEU

Samples:

NMRsample: AIDA1 SAM1, [U-98% 13C; U-98% 15N], 0.8 mM; D2O 10%; H2O 90%; sodium chloride 150 mM; sodium phosphate 20 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.8; pressure: 1.0 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNMRsampleisotropicsample_conditions_1
2D 1H-13C HSQCNMRsampleisotropicsample_conditions_1
3D CBCA(CO)NHNMRsampleisotropicsample_conditions_1
3D C(CO)NHNMRsampleisotropicsample_conditions_1
3D HNCONMRsampleisotropicsample_conditions_1
3D HNCACBNMRsampleisotropicsample_conditions_1
3D H(CCO)NHNMRsampleisotropicsample_conditions_1
3D 1H-15N NOESYNMRsampleisotropicsample_conditions_1
3D 1H-13C NOESYNMRsampleisotropicsample_conditions_1

Software:

X-PLOR NIH v2.21, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMRView v5, Johnson, One Moon Scientific - peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian NMRS 600 MHz

Related Database Links:

GB AY283057.2 AAF80756 AAH26313 AAH68451 AAH98373 AAI49112
PDB
DBJ BAE01854 BAG57507 BAI47262
EMBL CAH91693
REF NP_001095761 NP_001121558 NP_001125990 NP_001170868 NP_001190995
TPG DAA29568

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts