BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16140

Title: [Sec13,Sec14]-kappa-hexatoxin-Hv1c   PubMed: 19890933

Deposition date: 2009-01-27 Original release date: 2010-01-11

Authors: King, Glenn; Mobli, Mehdi

Citation: Mobli, Mehdi; de Araujo, Aline Dantas; Lambert, Lynette; Pierens, Gregory; Windley, Monique; Nicholson, Graham; Alewood, Paul; King, Glenn. "Direct visualization of disulfide bonds through diselenide proxies using 77Se NMR spectroscopy."  Angew. Chem. Int. Ed. Engl. 48, 9312-9314 (2009).

Assembly members:
kappa-hexatoxin-Hv1c, polymer, 37 residues, Formula weight is not available

Natural source:   Common Name: spider   Taxonomy ID: 6904   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hadronyche versuta

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
kappa-hexatoxin-Hv1c: AICTGADRPCAAXXPCCPGT SCKAESNGVSYCRKDEP

Data sets:
Data typeCount
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Monomer1

Entities:

Entity 1, Monomer 37 residues - Formula weight is not available

Residues 13 and 14 are selenocysteine

1   ALAILECYSTHRGLYALAASPARGPROCYS
2   ALAALASECSECPROCYSCYSPROGLYTHR
3   SERCYSLYSALAGLUSERASNGLYVALSER
4   TYRCYSARGLYSASPGLUPRO

Samples:

sample_1: kappa-hexatoxin-Hv1c 0.5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.01 M; pH: 4.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

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