BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16144

Title: Backbone and side chain 1H, 13C, and 15N NMR assignments for the organic hydroperoxide resistance protein (Ohr) from Burkholderia pseudomallei, Seattle Structural Genomics Center for Infectious Disease (SSGCID) target BupsA.00074   PubMed: 19888681

Deposition date: 2009-01-28 Original release date: 2009-01-30

Authors: Buchko, Garry; Hewitt, Stephen; Napuli, Alberto; Van Voorhis, Wesley; Myler, Peter

Citation: Buchko, Garry; Hewitt, Stephen; Napuli, Alberto; Van Voorhis, Wesley; Myler, Peter. "Backbone and side chain (1)H, (13)C, and (15)N NMR assignments for the organic hydroperoxide resistance protein (Ohr) from Burkholderia pseudomallei."  Biomol. NMR Assignments 3, 163-166 (2009).

Assembly members:
organic_hydroperoxide_resistance_protein_(Ohr), polymer, 160 residues, Formula weight is not available

Natural source:   Common Name: Burkholderia pseudomallei   Taxonomy ID: 28450   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia pseudomallei

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
organic_hydroperoxide_resistance_protein_(Ohr): MAHHHHHHMGTLEAQTQGPG SMNILYKTAATSTGGRDGRA TSHDQKLDVKLSAPRELGGA GAEGTNPEQLFAAGYSACFL SAMKFVAGQNKQTLPADTTV TAEVGIGPNEEGGFALDVEL RVALPGLDAAAAKTLVDRAH HVCPYSNATRNNVAVRLVVA

Data sets:
Data typeCount
13C chemical shifts517
15N chemical shifts139
1H chemical shifts782

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bp-Ohr, chain 11
2Bp-Ohr, chain 21

Entities:

Entity 1, Bp-Ohr, chain 1 160 residues - Formula weight is not available

The first N-terminal 21 residues (MAHHHHHHMGTLEAQTQGPGS-) are a non-native tag. The first residue in the native sequence is M22.

1   METALAHISHISHISHISHISHISMETGLY
2   THRLEUGLUALAGLNTHRGLNGLYPROGLY
3   SERMETASNILELEUTYRLYSTHRALAALA
4   THRSERTHRGLYGLYARGASPGLYARGALA
5   THRSERHISASPGLNLYSLEUASPVALLYS
6   LEUSERALAPROARGGLULEUGLYGLYALA
7   GLYALAGLUGLYTHRASNPROGLUGLNLEU
8   PHEALAALAGLYTYRSERALACYSPHELEU
9   SERALAMETLYSPHEVALALAGLYGLNASN
10   LYSGLNTHRLEUPROALAASPTHRTHRVAL
11   THRALAGLUVALGLYILEGLYPROASNGLU
12   GLUGLYGLYPHEALALEUASPVALGLULEU
13   ARGVALALALEUPROGLYLEUASPALAALA
14   ALAALALYSTHRLEUVALASPARGALAHIS
15   HISVALCYSPROTYRSERASNALATHRARG
16   ASNASNVALALAVALARGLEUVALVALALA

Samples:

sample_1: organic hydroperoxide resistance protein (Ohr), [U-99% 13C; U-99% 15N], 1.20 ± 0.2 mM; sodium chloride 100 ± 3 mM; DTT 1 ± 0.2 mM; TRIS 20 ± 1 mM

sample_2: organic hydroperoxide resistance protein (Ohr), [U-99% 13C; U-100% 15N; U-50% 2H], 1.20 ± 0.2 mM; sodium chloride 100 ± 3 mM; DTT 1 ± 0.2 mM; TRIS 20 ± 1 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1

Software:

FELIX v2007, Accelrys Software Inc. - processing

SPARKY v3.115, Goddard - data analysis

VNMR, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz
  • Varian INOVA 800 MHz

Related Database Links:

EMBL CAH39258 CDU31823 CFB51471 CFD89896 CFD92517
GB AAU46184 ABA51242 ABM49396 ABM99747 ABN87360
REF WP_004190960 WP_004529046 WP_004540142 WP_011832128 WP_038742729

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts