BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16147

Title: NMR assignments of oxidised thioredoxin from Plasmodium falciparum   PubMed: 19888680

Deposition date: 2009-01-29 Original release date: 2009-05-13

Authors: Munte, Claudia; Katja, Becker; Rolf, Schirmer; Hans, Kalbitzer

Citation: Munte, Claudia Elisabeth; Becker, Katja; Schirmer, Rolf Heiner; Kalbitzer, Hans Robert. "NMR assignments of oxidised thioredoxin from Plasmodium falciparum."  Biomol. NMR Assignments 3, 159-161 (2009).

Assembly members:
Trx, polymer, 115 residues, Formula weight is not available

Natural source:   Common Name: malaria parasite P. falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Trx: MRGSHHHHHHGSVKIVTSQS EFDSIISQNELVIVDFFAEW CGPCKRIAPFYEECSKTYTK MVFIKVDVDEVSEVTEKENI TSMPTFKVYKNGSSVDTLLG ANDSALKQLIEKYAA

Data sets:
Data typeCount
13C chemical shifts423
15N chemical shifts101
1H chemical shifts757

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Trx1

Entities:

Entity 1, Trx 115 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERVALLYSILEVALTHRSERGLNSER
3   GLUPHEASPSERILEILESERGLNASNGLU
4   LEUVALILEVALASPPHEPHEALAGLUTRP
5   CYSGLYPROCYSLYSARGILEALAPROPHE
6   TYRGLUGLUCYSSERLYSTHRTYRTHRLYS
7   METVALPHEILELYSVALASPVALASPGLU
8   VALSERGLUVALTHRGLULYSGLUASNILE
9   THRSERMETPROTHRPHELYSVALTYRLYS
10   ASNGLYSERSERVALASPTHRLEULEUGLY
11   ALAASNASPSERALALEULYSGLNLEUILE
12   GLULYSTYRALAALA

Samples:

sample_1: Trx, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 10 mM; DSS 0.1 mM; sodium azide 1 mM

sample_2: Trx, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 10 mM; DSS 0.1 mM; sodium azide 1 mM; D2O 8%

sample_3: Trx 1 mM; potassium phosphate 10 mM; DSS 0.1 mM; sodium azide 1 mM; D2O 8%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

AUREMOL, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAB90828 CDO67036
GB AAF34541 AAN37158 ETW15871 ETW28975 ETW33689
REF XP_001348719 XP_012765615
SP Q7KQL8

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