BMRB Entry 16174

Name: 15N, 13C and 1H resonance assignments for the Rv0287-Rv0288 complex
Published: 2009-07-22

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16174

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 15N, 13C and 1H resonance assignments for the Rv0287-Rv0288 complex PubMed: 19888683

Deposition date: 2009-02-13 Original release date: 2009-07-22

Authors: Ilghari, Dariush; Waters, Lorna; Veverka, Vaclave; Muskett, Frederick; Carr, Mark

Citation: Ilghari, Dariush; Waters, Lorna; Veverka, Vaclav; Muskett, Frederick; Carr, Mark. "(15)N, (13)C and (1)H resonance assignments and secondary structure determination of the Mycobacterium tuberculosis Rv0287-Rv0288 protein complex." Biomol. NMR Assignments 3, 171-174 (2009).

Assembly members:
Rv0287, polymer, 97 residues, 9777.91 Da.
Rv0288, polymer, 97 residues, 10477.6 Da.

Natural source: Common Name: tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Rv0287: MSLLDAHIPQLVASQSAFAA KAGLMRHTIGQAEQAAMSAQ AFHQGESSAAFQAAHARFVA AAAKVNTLLDVAQANLGEAA GTYVAADAAAASTYTGF
Rv0288: SMSQIMYNYPAMLGHAGDMA GYAGTLQSLGAEIAVEQAAL QSAWQGDTGITYQAWQAQWN QAMEDLVRAYHAMSSTHEAN TMAMMARDTAEAAKWGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shifts

Data type	Count
13C chemical shifts	487
15N chemical shifts	197
1H chemical shifts	1146

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	Rv0287	1
2	Rv0288	2

Samples:

sample_1: Rv0287, [U-98% 15N], .7 mM; Rv0288 .7 mM; sodium phosphate 25 mM; sodium chloride 100 mM; PMSF 100 uM; sodium azide .02%; H2O 90%; D2O 10%

sample_2: Rv0288, [U-98% 15N], .7 mM; Rv0287 .7 mM; sodium phosphate 25 mM; sodium chloride 100 mM; PMSF 100 uM; sodium azide .02%; H2O 90%; D2O 10%

sample_3: Rv0287, [U-99% 13C], 1 mM; Rv0288 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; PMSF 100 uM; sodium azide .02%; D2O 100%

sample_4: Rv0288, [U-99% 13C], 1 mM; Rv0287 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; PMSF 100 uM; sodium azide .02%; D2O 100%

sample_5: Rv0287, [U-99% 13C; U-98% 15N], 1 mM; Rv0288 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; PMSF 100 uM; sodium azide .02%; D2O 10%; H2O 90%

sample_6: Rv0288, [U-99% 13C; U-98% 15N], 1 mM; Rv0287 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; PMSF 100 uM; sodium azide .02%; D2O 10%; H2O 90%

sample_7: Rv0288 .7 mM; Rv0287 .7 mM; sodium phosphate 25 mM; sodium chloride 100 mM; PMSF 100 uM; sodium azide .02%; D2O 100%

sample_conditions: ionic strength: .125 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions
2D 1H-1H TOCSY	sample_7	isotropic	sample_conditions
2D 1H-1H NOESY	sample_7	isotropic	sample_conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions
3D HNCA	sample_5	isotropic	sample_conditions
3D HNCACB	sample_5	isotropic	sample_conditions
3D CBCA (CO) NH	sample_5	isotropic	sample_conditions
TROSY HNCOCA	sample_5	isotropic	sample_conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions
3D HCCH-COSY	sample_4	isotropic	sample_conditions
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions
3D HNCA	sample_6	isotropic	sample_conditions
3D HNCACB	sample_6	isotropic	sample_conditions
3D CBCA(CO)NH	sample_6	isotropic	sample_conditions
TROSY HNCOCA	sample_6	isotropic	sample_conditions

PDB	2KG7 2KG7
DBJ	BAH24594 BAL64138 BAQ04147 GAA44081 BAH24595 BAL64139 BAQ04148 GAA44082
EMBL	CAL70312 CCC25361 CCC42636 CCC62888 CCE35829 CAA05168 CAD93160 CAL70313 CCC25362 CCC42637
GB	AAK44524 ABQ72014 ABR04637 ACT23320 AEB02425 AAK44525 ABQ72015 ABR04638 ACT23321 AEB02426
REF	NP_214801 NP_853959 WP_003401503 WP_023643653 WP_031652217 NP_214802 NP_334711 NP_853960 WP_003401514 WP_003902934
SP	P0A569 P9WNK2 P9WNK3

Biological Magnetic Resonance Data Bank

BMRB Entry 16174

Title: 15N, 13C and 1H resonance assignments for the Rv0287-Rv0288 complex PubMed: 19888683

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