BMRB Entry 16216
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16216
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of protein ITSN1 from Homo sapiens. Northeast Structural Genomics Consortium target HR5524A
Deposition date: 2009-03-16 Original release date: 2009-04-23
Authors: Wu, Yibing; Ghosh, Arindam; Shastry, Ritu; Hua, Jia; Ciccosanti, Colleen; Zhang, Qi; Jiang, Mei; Swapna, G.V.T.; Acton, T.B.; Xiao, Rong; Everett, J.K.; Montelione, G.T.; SZYPERSKI, THOMAS
Citation: Wu, Yibing; Ghosh, Arindam; Shastry, Ritu; Hua, Jia; Ciccosanti, Colleen; Zhang, Qi; Jiang, Mei; Swapna, G.V.T.; Acton, T.B.; Xiao, Rong; Everett, J.K.; Montelione, G.T.; SZYPERSKI, THOMAS. "Solution structure of protein ITSN1 from Homo sapiens. Northeast Structural Genomics Consortium target HR5524A" Not known ., .-..
Assembly members:
ITSN1, polymer, 111 residues, 12614.523 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ITSN1: PPVAEWAVPQSSRLKYRQLF
NSHDKTMSGHLTGPQARTIL
MQSSLPQAQLASIWNLSDID
QDGKLTAEEFILAMHLIDVA
MSGQPLPPVLPPEYIPPSFR
RVRLEHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 360 |
15N chemical shifts | 103 |
1H chemical shifts | 749 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | ITSN1 | 1 |
Entities:
Entity 1, ITSN1 111 residues - 12614.523 Da.
1 | PRO | PRO | VAL | ALA | GLU | TRP | ALA | VAL | PRO | GLN | ||||
2 | SER | SER | ARG | LEU | LYS | TYR | ARG | GLN | LEU | PHE | ||||
3 | ASN | SER | HIS | ASP | LYS | THR | MET | SER | GLY | HIS | ||||
4 | LEU | THR | GLY | PRO | GLN | ALA | ARG | THR | ILE | LEU | ||||
5 | MET | GLN | SER | SER | LEU | PRO | GLN | ALA | GLN | LEU | ||||
6 | ALA | SER | ILE | TRP | ASN | LEU | SER | ASP | ILE | ASP | ||||
7 | GLN | ASP | GLY | LYS | LEU | THR | ALA | GLU | GLU | PHE | ||||
8 | ILE | LEU | ALA | MET | HIS | LEU | ILE | ASP | VAL | ALA | ||||
9 | MET | SER | GLY | GLN | PRO | LEU | PRO | PRO | VAL | LEU | ||||
10 | PRO | PRO | GLU | TYR | ILE | PRO | PRO | SER | PHE | ARG | ||||
11 | ARG | VAL | ARG | LEU | GLU | HIS | HIS | HIS | HIS | HIS | ||||
12 | HIS |
Samples:
sample_1: entity, [U-100% 13C; U-100% 15N], 1.0 mM; H2O 90%; D2O 10%
sample_2: entity, [U-5% 13C; U-99% 15N], 1.0 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
4,3D, GFT HNNCABCA | sample_1 | isotropic | sample_conditions_1 |
4,3D, GFT CABCACONNH | sample_1 | isotropic | sample_conditions_1 |
4,3D, GFT HCCH COSY | sample_1 | isotropic | sample_conditions_1 |
3D, 15N-13C RESOLVED SIMULTANEOUS NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment
AutoStruct, Huang, Tejero, Powers and Montelione - structure solution
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
TALOS, Cornilescu, Delaglio and Bax - structure solution
XEASY, Bartels et al. - peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 750 MHz
- Varian INOVA 600 MHz
Related Database Links:
PDB | |
EMBL | CAD98050 |
GB | ACE60215 ADF97294 ADF97295 ELW62068 EQB78493 |
REF | XP_006523000 XP_007962709 XP_008273162 XP_008944090 XP_009508108 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts