BMRB Entry 16230

Assembly:

Entities:

Entity 1, Prp24-RRM2 92 residues - 10012.586 Da.

Final 8 residues are non-native due to 6x His tag

Entity 2, AGAGAU 6 residues - 1826.235 Da.

Samples:

Unlabeled: Prp24-RRM2 300 uM; AGAGAU 3 mM; TRIS, [U-2H], 10 mM; potassium chloride 50 mM; D20 100%

Fully_Labeled_D2O: Prp24-RRM2, [U-99% 15N], 500 uM; AGAGAU 5 mM; TRIS, [U-2H], 10 mM; potassium chloride 50 mM; D20 100%

Fully_Labeled: Prp24-RRM2, [U-99% 13C; U-99% 15N], 500 mM; AGAGAU 5 mM; TRIS 10 mM; potassium chloride 50 mM; DTT 1 mM; H20 90%; D20 10%

Referencing: Prp24-RRM2, [U-99% 13C; U-99% 15N], 500 uM; AGAGAU 5 mM; TRIS 10 mM; potassium chloride 50 mM; DTT 1 mM; DSS 10 uM; H20 90%; D20 10%

Aligned: Prp24-RRM2, [U-99% 13C; U-99% 15N], 300 uM; AGAGAU 3 mM; TRIS 10 mM; potassium chloride 50 mM; DTT 1 mM; DMPC/DHPC 3:1 6.5%; H20 90%; D20 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1.0 atm; temperature: 298.15 K

Experiments:

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

ATHNOS-CANDID, Herrmann, T; Guentert, P; Wuethrich, K - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

TALOS, Cornilescu, Delaglio and Bax - data analysis

HADDOCK v2, Dominguez, C; Boelens, R; Bonvin, A M M J - refinement, structure solution

NMR spectrometers:

• Varian INOVA 600 MHz
• Varian DMX 750 MHz
• Varian INOVA 900 MHz
• Varian INOVA 800 MHz