BMRB Entry 16252
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16252
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Title: 1H, 13C, 15N Chemical shift assignments of A13 homeodomain PubMed: 19888690
Deposition date: 2009-04-10 Original release date: 2009-07-10
Authors: Ames, James
Citation: Zhang, Yonghong; Thornburg, Chelsea; Stadler, H. Scott; Ames, James. "(1)H, (15)N, and (13)C chemical shift assignments of mouse HOXA13 DNA binding domain." Biomol. NMR Assignments 3, 199-201 (2009).
Assembly members:
A13, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
A13: MSHMLEGRKKRVPYTKVQLK
ELEREYATNKFITKDKRRRI
SATTNLSERQVTIWFQNRRV
KEKKVINKLKTTS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 232 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 486 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | A13 | 1 |
Entities:
Entity 1, A13 73 residues - Formula weight is not available
Residues 1-6 are due to a cloning artifact.
| 1 | MET | SER | HIS | MET | LEU | GLU | GLY | ARG | LYS | LYS | ||||
| 2 | ARG | VAL | PRO | TYR | THR | LYS | VAL | GLN | LEU | LYS | ||||
| 3 | GLU | LEU | GLU | ARG | GLU | TYR | ALA | THR | ASN | LYS | ||||
| 4 | PHE | ILE | THR | LYS | ASP | LYS | ARG | ARG | ARG | ILE | ||||
| 5 | SER | ALA | THR | THR | ASN | LEU | SER | GLU | ARG | GLN | ||||
| 6 | VAL | THR | ILE | TRP | PHE | GLN | ASN | ARG | ARG | VAL | ||||
| 7 | LYS | GLU | LYS | LYS | VAL | ILE | ASN | LYS | LEU | LYS | ||||
| 8 | THR | THR | SER |
Samples:
sample_1: A13, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.02 M; pH: 7.0; pressure: 1.0 atm; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 16577 |
| PDB | |
| EMBL | CAC44975 |
| GB | AAA86506 AAB03322 AAC50993 AAH75791 AAI00732 |
| REF | NP_000513 NP_001079321 NP_001162372 NP_001182271 NP_001258284 |
| SP | P31271 P50210 Q62424 Q90X25 |
| TPG | DAA30521 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts