BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16262

Title: NMR solution structure of the N-terminal domain of the DNA polymerase alpha p68 subunit

Deposition date: 2009-04-22 Original release date: 2012-08-06

Authors: Huang, Hao; Weiner, Brian; Zhang, Haijiang; Fuller, Brian; Gao, Yue; Wile, Brian; Chazin, Walter; Fanning, Ellen

Citation: Huang, Hao; Weiner, Brian; Zhang, Haijiang; Fuller, Brian; Gao, Yue; Wile, Brian; Chazin, Walter; Fanning, Ellen. "The N-terminal domain of the p68 subunit tethers DNA polymerase alpha-primase to a replicative helicase for primosome function"  Not known ., .-..

Assembly members:
p68N, polymer, 101 residues, 8700.943 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
p68N: MGSSHHHHHHGSSLEVLFQG PGSMSASAQQLAEELQIFGL DCEEALIEKLVELCVQYGQN EEGMVGELIAFCTSTHKVGL TSEILNSFEHEFLSKRLSKA R

Data sets:
Data typeCount
13C chemical shifts235
15N chemical shifts83
1H chemical shifts550

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1p68N1

Entities:

Entity 1, p68N 101 residues - 8700.943 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   GLYSERSERLEUGLUVALLEUPHEGLNGLY
3   PROGLYSERMETSERALASERALAGLNGLN
4   LEUALAGLUGLULEUGLNILEPHEGLYLEU
5   ASPCYSGLUGLUALALEUILEGLULYSLEU
6   VALGLULEUCYSVALGLNTYRGLYGLNASN
7   GLUGLUGLYMETVALGLYGLULEUILEALA
8   PHECYSTHRSERTHRHISLYSVALGLYLEU
9   THRSERGLUILELEUASNSERPHEGLUHIS
10   GLUPHELEUSERLYSARGLEUSERLYSALA
11   ARG

Samples:

sample_1: p68N, [U-100% 13C; U-100% 15N], 0.75 mM; NACL 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CC)(CO)NH TOCSYsample_1isotropicsample_conditions_1
3D (H)CC(CO)NH TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, structure solution

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAG73551
EMBL CAG33018
GB AAA16459 AAH01347 AAH02990 ABM82949 ABM86141
REF NP_001253937 NP_002680 XP_001169495 XP_003828668 XP_003909632
SP Q14181

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts