BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16271

Title: A PH domain within OCRL bridges clathrin mediated membrane trafficking to phosphoinositide metabolis   PubMed: 19536138

Deposition date: 2009-05-03 Original release date: 2009-06-25

Authors: Mao, Yuxin; Balkin, Daniel; Zoncu, Roberto; Erdmann, Kai; Tomasini, Livia; Hu, Fenghua; Jin, Moonsoon; Hodsdon, Michael; De Camilli, Pietro

Citation: Mao, Yuxin; Balkin, Daniel; Zoncu, Roberto; Erdmann, Kai; Tomasini, Livia; Hu, Fenghua; Jin, Moonsoon; Hodsdon, Michael; De Camilli, Pietro. "A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism"  EMBO J. 28, 1831-1842 (2009).

Assembly members:
PH domain, polymer, 372 residues, 41573.961 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PH domain: GPLGSMEPPLPVGAQPLATV EGMEMKGPLREPCALTLAQR NGQYELIIQLHEKEQHVQDI IPINSHFRCVQEAEETLLID IASNSGCKIRVQGDWIRERR FEIPDEEHCLKFLSAVLAAQ KAQSGPLGSMEPPLPVGAQP LATVEGMEMKGPLREPCALT LAQRNGQYELIIQLHEKEQH VQDIIPINSHFRCVQEAEET LLIDIASNSGCKIRVQGDWI RERRFEIPDEEHCLKFLSAV LAAQKAQSGPLGSMEPPLPV GAQPLATVEGMEMKGPLREP CALTLAQRNGQYELIIQLHE KEQHVQDIIPINSHFRCVQE AEETLLIDIASNSGCKIRVQ GDWIRERRFEIPDEEHCLKF LSAVLAAQKAQS

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts131
1H chemical shifts899

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PH domain1

Entities:

Entity 1, PH domain 372 residues - 41573.961 Da.

1   GLYPROLEUGLYSERMETGLUPROPROLEU
2   PROVALGLYALAGLNPROLEUALATHRVAL
3   GLUGLYMETGLUMETLYSGLYPROLEUARG
4   GLUPROCYSALALEUTHRLEUALAGLNARG
5   ASNGLYGLNTYRGLULEUILEILEGLNLEU
6   HISGLULYSGLUGLNHISVALGLNASPILE
7   ILEPROILEASNSERHISPHEARGCYSVAL
8   GLNGLUALAGLUGLUTHRLEULEUILEASP
9   ILEALASERASNSERGLYCYSLYSILEARG
10   VALGLNGLYASPTRPILEARGGLUARGARG
11   PHEGLUILEPROASPGLUGLUHISCYSLEU
12   LYSPHELEUSERALAVALLEUALAALAGLN
13   LYSALAGLNSERGLYPROLEUGLYSERMET
14   GLUPROPROLEUPROVALGLYALAGLNPRO
15   LEUALATHRVALGLUGLYMETGLUMETLYS
16   GLYPROLEUARGGLUPROCYSALALEUTHR
17   LEUALAGLNARGASNGLYGLNTYRGLULEU
18   ILEILEGLNLEUHISGLULYSGLUGLNHIS
19   VALGLNASPILEILEPROILEASNSERHIS
20   PHEARGCYSVALGLNGLUALAGLUGLUTHR
21   LEULEUILEASPILEALASERASNSERGLY
22   CYSLYSILEARGVALGLNGLYASPTRPILE
23   ARGGLUARGARGPHEGLUILEPROASPGLU
24   GLUHISCYSLEULYSPHELEUSERALAVAL
25   LEUALAALAGLNLYSALAGLNSERGLYPRO
26   LEUGLYSERMETGLUPROPROLEUPROVAL
27   GLYALAGLNPROLEUALATHRVALGLUGLY
28   METGLUMETLYSGLYPROLEUARGGLUPRO
29   CYSALALEUTHRLEUALAGLNARGASNGLY
30   GLNTYRGLULEUILEILEGLNLEUHISGLU
31   LYSGLUGLNHISVALGLNASPILEILEPRO
32   ILEASNSERHISPHEARGCYSVALGLNGLU
33   ALAGLUGLUTHRLEULEUILEASPILEALA
34   SERASNSERGLYCYSLYSILEARGVALGLN
35   GLYASPTRPILEARGGLUARGARGPHEGLU
36   ILEPROASPGLUGLUHISCYSLEULYSPHE
37   LEUSERALAVALLEUALAALAGLNLYSALA
38   GLNSER

Samples:

sample_1: entity 1 mM; potassium phosphate 20 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization

NMR spectrometers:

  • Varian INOVA 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts