BMRB Entry 16272

Name: Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.
Published: 2010-03-09

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PDB ID: 2kif
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16272
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.

Deposition date: 2009-05-03 Original release date: 2010-03-09

Authors: Aramini, James; Belote, Rachel; Ciccosanti, Colleen; Jiang, Mei; Rost, Burkhard; Nair, Rajesh; Swapna, G.; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano

Citation: Aramini, James; Belote, Rachel; Ciccosanti, Colleen; Jiang, Mei; Rost, Burkhard; Nair, Rajesh; Swapna, G.; Acton, Thomas; Xiao, Rong; Everett, John; Montelione, Gaetano. "Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247." Not known ., .-..

Assembly members:
VpR247, polymer, 108 residues, 12341.439 Da.

Natural source: Common Name: Vibrio parahaemolyticus Taxonomy ID: 670 Superkingdom: Bacteria Kingdom: not available Genus/species: Vibrio parahaemolyticus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
VpR247: MDQFLVQIFAVIHQIPKGKV STYGEIAKMAGYPGYARHVG KALGNLPEGSKLPWFRVINS QGKISLKGRDLDRQKQKLEA EGIEVSEIGKIALRKYKWQP LEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	486
15N chemical shifts	104
1H chemical shifts	768

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D simultaneous CN NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY aliphatic	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY aliphatic	sample_2	isotropic	sample_conditions_1
3D (H)CCH-TOCSY aliphatic	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1
2D 1H-15N hetNOE	sample_2	isotropic	sample_conditions_1
1D 1H-15N T1 and T2	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC high res. (L/V methyl stereoassignment)	sample_3	isotropic	sample_conditions_1

BMRB	16283
PDB	2KIF 2KIM
DBJ	BAC59214
GB	AGB09412 AGQ90485 AGQ98830 AHI99009 AKU55569
REF	NP_797330 WP_005461131 WP_005481834 WP_021449579 WP_025501692
SP	A6B4U8

Biological Magnetic Resonance Data Bank

BMRB Entry 16272

Title: Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.

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